Laryngeal abductor muscle reinnervation in a pig model

MA Birchall, B Idowu, PJ Murison, A Jones, RM Burt, SM Ayling, CR Stokes, L Pope, G Terenghi

Research output: Contribution to journalArticle (Academic Journal)peer-review

12 Citations (Scopus)

Abstract

Objective-To develop a large animal model for studies of laryngeal abductor reinnervation. Material and Methods-Six minipigs underwent unilateral anastomosis of the phrenic nerve-abductor branch of the recurrent laryngeal nerve (RLN). Polyhydroxybutyrate (PHB) conduits were used for repair. At each of 30, 60 and 120 days, 2 animals underwent video laryngeal endoscopy (VLE) and were then killed. VLE was also performed in the 120-day pair at 60 days. Nerve-conduit-nerve-muscle samples were fixed for light and immunofluorescence (pan-neurofilaments, S-100) microscopy. Laryngeal muscles were harvested (myosin heavy chain analysis). Results-VLE showed recovery of abductor function in 1 animal at 60 days and in 1 at 120 days. Haematoxylin-eosin staining demonstrated a complex inflammatory response. Eosinophil recruitment was observed. Stepwise regeneration and reorganization of the distal nerve between 30 and 120 days was observed with pan-NF staining. The mean minimum diameter in the reinnervated posterior crico-arytenoids tended to increase for up to 120 days. Conclusions-Anastomosis of the phrenic nerve-abductor branch of the RLN with a PHB conduit in a pig can result in functional and histological recovery within 2-4 months and appears to at least sustain abductor muscle fibre morphology. Recovery occurs despite a complex inflammatory response, which may be an essential part of healing rather than inhibitory.
Translated title of the contributionLaryngeal abductor muscle reinnervation in a pig model
Original languageEnglish
Pages (from-to)839 - 846
Number of pages7
JournalActa Otolaryngologica
Volume124 (7)
DOIs
Publication statusPublished - Sep 2004

Bibliographical note

Publisher: Taylor & Francis

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