TY - JOUR
T1 - Layered double hydroxide nanoparticles
T2 - Impact on vascular cells, blood cells and the complement system
AU - Gu, Zi
AU - Yan, Shiyu
AU - Cheong, Soshan
AU - Cao, Zhenbang
AU - Zuo, Huali
AU - Thomas, Anita C.
AU - Rolfe, Barbara E.
AU - Xu, Zhi Ping
PY - 2018/2/15
Y1 - 2018/2/15
N2 - The mounting interest in layered double hydroxide (LDH) nanoparticles as drug carriers and bio-imaging contrast agents makes biosafety evaluation of LDH essential. Considering the important role of blood circulation in bio-distribution of nanoparticles, the present work evaluated the impact of MgAl-LDHs on key components of the circulatory system, including vascular cells (vascular smooth muscle cells (SMCs) and endothelial cells (HUVECs)), red blood cells (RBCs), and complement activation. The results showed that LDH had no effects on SMCs and HUVECs at concentrations up to 500 and 10 µg/mL respectively, in terms of cell proliferation and viability. LDH (10 µg/mL) did not change either the migration distance or the number of migrating SMCs in culture. Moreover, LDH (400 µg/mL) had a negligible effect on RBCs’ lysis, and there was no significant increase in levels of complement activation product, C5a, in the presence of LDH (20 or 200 µg/mL). The low toxicity for vascular cells and blood cells combined with low immunogenicity sheds a light on the biosafety of LDH nanoparticles, and encourages further studies into their biomedical applications.
AB - The mounting interest in layered double hydroxide (LDH) nanoparticles as drug carriers and bio-imaging contrast agents makes biosafety evaluation of LDH essential. Considering the important role of blood circulation in bio-distribution of nanoparticles, the present work evaluated the impact of MgAl-LDHs on key components of the circulatory system, including vascular cells (vascular smooth muscle cells (SMCs) and endothelial cells (HUVECs)), red blood cells (RBCs), and complement activation. The results showed that LDH had no effects on SMCs and HUVECs at concentrations up to 500 and 10 µg/mL respectively, in terms of cell proliferation and viability. LDH (10 µg/mL) did not change either the migration distance or the number of migrating SMCs in culture. Moreover, LDH (400 µg/mL) had a negligible effect on RBCs’ lysis, and there was no significant increase in levels of complement activation product, C5a, in the presence of LDH (20 or 200 µg/mL). The low toxicity for vascular cells and blood cells combined with low immunogenicity sheds a light on the biosafety of LDH nanoparticles, and encourages further studies into their biomedical applications.
KW - Biosafety
KW - Cell migration
KW - Cell proliferation and viability
KW - Complement activation
KW - Haemolysis
KW - Layered double hydroxide
KW - Vascular cells
UR - http://www.scopus.com/inward/record.url?scp=85032194229&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2017.10.069
DO - 10.1016/j.jcis.2017.10.069
M3 - Article (Academic Journal)
C2 - 29096100
AN - SCOPUS:85032194229
VL - 512
SP - 404
EP - 410
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
SN - 0021-9797
ER -