Layered double hydroxide nanoparticles: Impact on vascular cells, blood cells and the complement system

Zi Gu*, Shiyu Yan, Soshan Cheong, Zhenbang Cao, Huali Zuo, Anita C. Thomas, Barbara E. Rolfe, Zhi Ping Xu

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

15 Citations (Scopus)
312 Downloads (Pure)

Abstract

The mounting interest in layered double hydroxide (LDH) nanoparticles as drug carriers and bio-imaging contrast agents makes biosafety evaluation of LDH essential. Considering the important role of blood circulation in bio-distribution of nanoparticles, the present work evaluated the impact of MgAl-LDHs on key components of the circulatory system, including vascular cells (vascular smooth muscle cells (SMCs) and endothelial cells (HUVECs)), red blood cells (RBCs), and complement activation. The results showed that LDH had no effects on SMCs and HUVECs at concentrations up to 500 and 10 µg/mL respectively, in terms of cell proliferation and viability. LDH (10 µg/mL) did not change either the migration distance or the number of migrating SMCs in culture. Moreover, LDH (400 µg/mL) had a negligible effect on RBCs’ lysis, and there was no significant increase in levels of complement activation product, C5a, in the presence of LDH (20 or 200 µg/mL). The low toxicity for vascular cells and blood cells combined with low immunogenicity sheds a light on the biosafety of LDH nanoparticles, and encourages further studies into their biomedical applications.

Original languageEnglish
Pages (from-to)404-410
Number of pages7
JournalJournal of Colloid and Interface Science
Volume512
Early online date19 Oct 2017
DOIs
Publication statusPublished - 15 Feb 2018

Keywords

  • Biosafety
  • Cell migration
  • Cell proliferation and viability
  • Complement activation
  • Haemolysis
  • Layered double hydroxide
  • Vascular cells

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