Levels of blood CD1c+ mDC1 and CD1chi mDC1 subpopulation reflect disease activity in noninfectious uveitis

Ping Chen; William Tucker; Susan Hannes; Baoying Liu; Han Si; Ankur Gupta; Richard W J Lee; H Nida Sen; Robert B Nussenblatt

doi:10.1167/iovs.14-15416

Levels of blood CD1c+ mDC1 and CD1chi mDC1 subpopulation reflect disease activity in noninfectious uveitis

Ping Chen, William Tucker, Susan Hannes, Baoying Liu, Han Si, Ankur Gupta, Richard W J Lee, H Nida Sen, Robert B Nussenblatt

Research output: Contribution to journal › Article (Academic Journal) › peer-review

9 Citations (Scopus)

Abstract

PURPOSE: Myeloid dendritic cells (mDCs) play an important role in autoimmune diseases. However, the role of blood CD1c(+) myeloid dendritic cells 1 (mDC1s), the subset of human blood mDCs, is not well understood in noninfectious uveitis.

METHODS: Fresh peripheral blood samples from human noninfectious uveitis patients (n = 32) and healthy controls (HCs) (n = 64) were stained with FITC-Lineage 1 (Lin1), PERCP-HLADR, and PE-CD1c antibodies. The levels of mDC1 were quantified by using flow cytometric analysis. Longitudinal data from patients (n = 16) were analyzed to correlate the levels of mDC1 with disease activity.

RESULTS: Blood CD1c(+) mDC1 and its subpopulation, CD1c(hi) mDC1, were increased in uveitis patients compared with HCs. Longitudinal data demonstrated that both the CD1c(+) mDC1 and CD1c(hi) mDC1 subpopulation reflected a dynamic change in clinical uveitis activity: CD1c expression was increased in active uveitis but decreased when uveitis became inactive.

CONCLUSIONS: Given these observations, an alteration in blood CD1c(+) mDC1 and the CD1c(hi) mDC1 subpopulation could be a potential biomarker to monitor clinical uveitis activity within patients.

Original language	English
Pages (from-to)	346-52
Number of pages	7
Journal	Investigative Ophthalmology and Visual Science
Volume	56
Issue number	1
DOIs	https://doi.org/10.1167/iovs.14-15416
Publication status	Published - Jan 2015

Keywords

Adolescent
Adult
Aged
Antigens, CD1
Autoimmune Diseases
Biomarkers
Child
Dendritic Cells
Female
Flow Cytometry
Glycoproteins
Humans
Immunity, Cellular
Male
Middle Aged
Myeloid Cells
Nuclear Proteins
Trans-Activators
Uveitis
Young Adult

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{30ce08e1c0794d408fa9a405ec067e9d,

title = "Levels of blood CD1c+ mDC1 and CD1chi mDC1 subpopulation reflect disease activity in noninfectious uveitis",

abstract = "PURPOSE: Myeloid dendritic cells (mDCs) play an important role in autoimmune diseases. However, the role of blood CD1c(+) myeloid dendritic cells 1 (mDC1s), the subset of human blood mDCs, is not well understood in noninfectious uveitis.METHODS: Fresh peripheral blood samples from human noninfectious uveitis patients (n = 32) and healthy controls (HCs) (n = 64) were stained with FITC-Lineage 1 (Lin1), PERCP-HLADR, and PE-CD1c antibodies. The levels of mDC1 were quantified by using flow cytometric analysis. Longitudinal data from patients (n = 16) were analyzed to correlate the levels of mDC1 with disease activity.RESULTS: Blood CD1c(+) mDC1 and its subpopulation, CD1c(hi) mDC1, were increased in uveitis patients compared with HCs. Longitudinal data demonstrated that both the CD1c(+) mDC1 and CD1c(hi) mDC1 subpopulation reflected a dynamic change in clinical uveitis activity: CD1c expression was increased in active uveitis but decreased when uveitis became inactive.CONCLUSIONS: Given these observations, an alteration in blood CD1c(+) mDC1 and the CD1c(hi) mDC1 subpopulation could be a potential biomarker to monitor clinical uveitis activity within patients.",

keywords = "Adolescent, Adult, Aged, Antigens, CD1, Autoimmune Diseases, Biomarkers, Child, Dendritic Cells, Female, Flow Cytometry, Glycoproteins, Humans, Immunity, Cellular, Male, Middle Aged, Myeloid Cells, Nuclear Proteins, Trans-Activators, Uveitis, Young Adult",

author = "Ping Chen and William Tucker and Susan Hannes and Baoying Liu and Han Si and Ankur Gupta and Lee, {Richard W J} and Sen, {H Nida} and Nussenblatt, {Robert B}",

year = "2015",

month = jan,

doi = "10.1167/iovs.14-15416",

language = "English",

volume = "56",

pages = "346--52",

journal = "Investigative Ophthalmology and Visual Science",

issn = "0146-0404",

publisher = "Association for Research in Vision and Ophthalmology Inc.",

number = "1",

}

TY - JOUR

T1 - Levels of blood CD1c+ mDC1 and CD1chi mDC1 subpopulation reflect disease activity in noninfectious uveitis

AU - Chen, Ping

AU - Tucker, William

AU - Hannes, Susan

AU - Liu, Baoying

AU - Si, Han

AU - Gupta, Ankur

AU - Lee, Richard W J

AU - Sen, H Nida

AU - Nussenblatt, Robert B

PY - 2015/1

Y1 - 2015/1

N2 - PURPOSE: Myeloid dendritic cells (mDCs) play an important role in autoimmune diseases. However, the role of blood CD1c(+) myeloid dendritic cells 1 (mDC1s), the subset of human blood mDCs, is not well understood in noninfectious uveitis.METHODS: Fresh peripheral blood samples from human noninfectious uveitis patients (n = 32) and healthy controls (HCs) (n = 64) were stained with FITC-Lineage 1 (Lin1), PERCP-HLADR, and PE-CD1c antibodies. The levels of mDC1 were quantified by using flow cytometric analysis. Longitudinal data from patients (n = 16) were analyzed to correlate the levels of mDC1 with disease activity.RESULTS: Blood CD1c(+) mDC1 and its subpopulation, CD1c(hi) mDC1, were increased in uveitis patients compared with HCs. Longitudinal data demonstrated that both the CD1c(+) mDC1 and CD1c(hi) mDC1 subpopulation reflected a dynamic change in clinical uveitis activity: CD1c expression was increased in active uveitis but decreased when uveitis became inactive.CONCLUSIONS: Given these observations, an alteration in blood CD1c(+) mDC1 and the CD1c(hi) mDC1 subpopulation could be a potential biomarker to monitor clinical uveitis activity within patients.

AB - PURPOSE: Myeloid dendritic cells (mDCs) play an important role in autoimmune diseases. However, the role of blood CD1c(+) myeloid dendritic cells 1 (mDC1s), the subset of human blood mDCs, is not well understood in noninfectious uveitis.METHODS: Fresh peripheral blood samples from human noninfectious uveitis patients (n = 32) and healthy controls (HCs) (n = 64) were stained with FITC-Lineage 1 (Lin1), PERCP-HLADR, and PE-CD1c antibodies. The levels of mDC1 were quantified by using flow cytometric analysis. Longitudinal data from patients (n = 16) were analyzed to correlate the levels of mDC1 with disease activity.RESULTS: Blood CD1c(+) mDC1 and its subpopulation, CD1c(hi) mDC1, were increased in uveitis patients compared with HCs. Longitudinal data demonstrated that both the CD1c(+) mDC1 and CD1c(hi) mDC1 subpopulation reflected a dynamic change in clinical uveitis activity: CD1c expression was increased in active uveitis but decreased when uveitis became inactive.CONCLUSIONS: Given these observations, an alteration in blood CD1c(+) mDC1 and the CD1c(hi) mDC1 subpopulation could be a potential biomarker to monitor clinical uveitis activity within patients.

KW - Adolescent

KW - Adult

KW - Aged

KW - Antigens, CD1

KW - Autoimmune Diseases

KW - Biomarkers

KW - Child

KW - Dendritic Cells

KW - Female

KW - Flow Cytometry

KW - Glycoproteins

KW - Humans

KW - Immunity, Cellular

KW - Male

KW - Middle Aged

KW - Myeloid Cells

KW - Nuclear Proteins

KW - Trans-Activators

KW - Uveitis

KW - Young Adult

U2 - 10.1167/iovs.14-15416

DO - 10.1167/iovs.14-15416

M3 - Article (Academic Journal)

C2 - 25515573

SN - 0146-0404

VL - 56

SP - 346

EP - 352

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

IS - 1

ER -

Levels of blood CD1c+ mDC1 and CD1chi mDC1 subpopulation reflect disease activity in noninfectious uveitis

Abstract

Keywords

UN SDGs

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