Levels of blood CD1c+ mDC1 and CD1chi mDC1 subpopulation reflect disease activity in noninfectious uveitis

Ping Chen, William Tucker, Susan Hannes, Baoying Liu, Han Si, Ankur Gupta, Richard W J Lee, H Nida Sen, Robert B Nussenblatt

Research output: Contribution to journalArticle (Academic Journal)

5 Citations (Scopus)

Abstract

PURPOSE: Myeloid dendritic cells (mDCs) play an important role in autoimmune diseases. However, the role of blood CD1c(+) myeloid dendritic cells 1 (mDC1s), the subset of human blood mDCs, is not well understood in noninfectious uveitis.

METHODS: Fresh peripheral blood samples from human noninfectious uveitis patients (n = 32) and healthy controls (HCs) (n = 64) were stained with FITC-Lineage 1 (Lin1), PERCP-HLADR, and PE-CD1c antibodies. The levels of mDC1 were quantified by using flow cytometric analysis. Longitudinal data from patients (n = 16) were analyzed to correlate the levels of mDC1 with disease activity.

RESULTS: Blood CD1c(+) mDC1 and its subpopulation, CD1c(hi) mDC1, were increased in uveitis patients compared with HCs. Longitudinal data demonstrated that both the CD1c(+) mDC1 and CD1c(hi) mDC1 subpopulation reflected a dynamic change in clinical uveitis activity: CD1c expression was increased in active uveitis but decreased when uveitis became inactive.

CONCLUSIONS: Given these observations, an alteration in blood CD1c(+) mDC1 and the CD1c(hi) mDC1 subpopulation could be a potential biomarker to monitor clinical uveitis activity within patients.

Original languageEnglish
Pages (from-to)346-52
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume56
Issue number1
DOIs
Publication statusPublished - Jan 2015

Keywords

  • Adolescent
  • Adult
  • Aged
  • Antigens, CD1
  • Autoimmune Diseases
  • Biomarkers
  • Child
  • Dendritic Cells
  • Female
  • Flow Cytometry
  • Glycoproteins
  • Humans
  • Immunity, Cellular
  • Male
  • Middle Aged
  • Myeloid Cells
  • Nuclear Proteins
  • Trans-Activators
  • Uveitis
  • Young Adult

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