Lipid membrane curvature induced by distearoyl phosphatidylinositol 4-phosphate

Samuel Furse, Nicholas J. Brooks, Annela M. Seddon, Ruediger Woscholski, Richard H. Templer, Edward W. Tate, Piers R. J. Gaffney, Oscar Ces*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

26 Citations (Scopus)

Abstract

In this paper we present evidence that phosphatidylinositol 4-phosphate induces curvature in biological membranes. The phase behaviour of mixtures of distearoylphosphatidylinositol 4-phosphate (DSPIP) and dioleoylphosphatidylcholine (DOPC) as a function of pressure and temperature has been studied using small-angle X-ray scattering and in the presence of biologically relevant magnesium concentrations. Our results demonstrate that at physiologically relevant concentrations (2 mol%), DSPIP is capable of inducing the formation of the inverse hexagonal phase (H-II) over a wide range of conditions. This result has implications for the structural role of phosphatidylinositol lipids in vivo.

Original languageEnglish
Pages (from-to)3090-3093
Number of pages4
JournalSoft Matter
Volume8
Issue number11
DOIs
Publication statusPublished - 2012

Keywords

  • 3,4,5-TRISPHOSPHATE
  • HIGH-PRESSURE
  • ORGANIZATION
  • INOSITOL TRISPHOSPHATE
  • IDENTIFICATION
  • PHOSPHOINOSITIDES
  • X-RAY
  • PHASE-TRANSITIONS
  • DOMAINS
  • CLATHRIN-DEPENDENT ENDOCYTOSIS

Cite this

Furse, S., Brooks, N. J., Seddon, A. M., Woscholski, R., Templer, R. H., Tate, E. W., Gaffney, P. R. J., & Ces, O. (2012). Lipid membrane curvature induced by distearoyl phosphatidylinositol 4-phosphate. Soft Matter, 8(11), 3090-3093. https://doi.org/10.1039/c2sm07358g