Lipid membrane curvature induced by distearoyl phosphatidylinositol 4-phosphate

Samuel Furse; Nicholas J. Brooks; Annela M. Seddon; Ruediger Woscholski; Richard H. Templer; Edward W. Tate; Piers R. J. Gaffney; Oscar Ces

doi:10.1039/c2sm07358g

Lipid membrane curvature induced by distearoyl phosphatidylinositol 4-phosphate

Samuel Furse, Nicholas J. Brooks, Annela M. Seddon, Ruediger Woscholski, Richard H. Templer, Edward W. Tate, Piers R. J. Gaffney, Oscar Ces^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

38 Citations (Scopus)

Abstract

In this paper we present evidence that phosphatidylinositol 4-phosphate induces curvature in biological membranes. The phase behaviour of mixtures of distearoylphosphatidylinositol 4-phosphate (DSPIP) and dioleoylphosphatidylcholine (DOPC) as a function of pressure and temperature has been studied using small-angle X-ray scattering and in the presence of biologically relevant magnesium concentrations. Our results demonstrate that at physiologically relevant concentrations (2 mol%), DSPIP is capable of inducing the formation of the inverse hexagonal phase (H-II) over a wide range of conditions. This result has implications for the structural role of phosphatidylinositol lipids in vivo.

Original language	English
Pages (from-to)	3090-3093
Number of pages	4
Journal	Soft Matter
Volume	8
Issue number	11
DOIs	https://doi.org/10.1039/c2sm07358g
Publication status	Published - 2012

Keywords

3,4,5-TRISPHOSPHATE
HIGH-PRESSURE
ORGANIZATION
INOSITOL TRISPHOSPHATE
IDENTIFICATION
PHOSPHOINOSITIDES
X-RAY
PHASE-TRANSITIONS
DOMAINS
CLATHRIN-DEPENDENT ENDOCYTOSIS

Access to Document

10.1039/c2sm07358g

Handle.net

Persistent link

Cite this

@article{113faa14b5454ecf9af1812091a99fd0,

title = "Lipid membrane curvature induced by distearoyl phosphatidylinositol 4-phosphate",

abstract = "In this paper we present evidence that phosphatidylinositol 4-phosphate induces curvature in biological membranes. The phase behaviour of mixtures of distearoylphosphatidylinositol 4-phosphate (DSPIP) and dioleoylphosphatidylcholine (DOPC) as a function of pressure and temperature has been studied using small-angle X-ray scattering and in the presence of biologically relevant magnesium concentrations. Our results demonstrate that at physiologically relevant concentrations (2 mol\%), DSPIP is capable of inducing the formation of the inverse hexagonal phase (H-II) over a wide range of conditions. This result has implications for the structural role of phosphatidylinositol lipids in vivo.",

keywords = "3,4,5-TRISPHOSPHATE, HIGH-PRESSURE, ORGANIZATION, INOSITOL TRISPHOSPHATE, IDENTIFICATION, PHOSPHOINOSITIDES, X-RAY, PHASE-TRANSITIONS, DOMAINS, CLATHRIN-DEPENDENT ENDOCYTOSIS",

author = "Samuel Furse and Brooks, \{Nicholas J.\} and Seddon, \{Annela M.\} and Ruediger Woscholski and Templer, \{Richard H.\} and Tate, \{Edward W.\} and Gaffney, \{Piers R. J.\} and Oscar Ces",

year = "2012",

doi = "10.1039/c2sm07358g",

language = "English",

volume = "8",

pages = "3090--3093",

journal = "Soft Matter",

issn = "1744-683X",

publisher = "Royal Society of Chemistry",

number = "11",

}

TY - JOUR

T1 - Lipid membrane curvature induced by distearoyl phosphatidylinositol 4-phosphate

AU - Furse, Samuel

AU - Brooks, Nicholas J.

AU - Seddon, Annela M.

AU - Woscholski, Ruediger

AU - Templer, Richard H.

AU - Tate, Edward W.

AU - Gaffney, Piers R. J.

AU - Ces, Oscar

PY - 2012

Y1 - 2012

N2 - In this paper we present evidence that phosphatidylinositol 4-phosphate induces curvature in biological membranes. The phase behaviour of mixtures of distearoylphosphatidylinositol 4-phosphate (DSPIP) and dioleoylphosphatidylcholine (DOPC) as a function of pressure and temperature has been studied using small-angle X-ray scattering and in the presence of biologically relevant magnesium concentrations. Our results demonstrate that at physiologically relevant concentrations (2 mol%), DSPIP is capable of inducing the formation of the inverse hexagonal phase (H-II) over a wide range of conditions. This result has implications for the structural role of phosphatidylinositol lipids in vivo.

AB - In this paper we present evidence that phosphatidylinositol 4-phosphate induces curvature in biological membranes. The phase behaviour of mixtures of distearoylphosphatidylinositol 4-phosphate (DSPIP) and dioleoylphosphatidylcholine (DOPC) as a function of pressure and temperature has been studied using small-angle X-ray scattering and in the presence of biologically relevant magnesium concentrations. Our results demonstrate that at physiologically relevant concentrations (2 mol%), DSPIP is capable of inducing the formation of the inverse hexagonal phase (H-II) over a wide range of conditions. This result has implications for the structural role of phosphatidylinositol lipids in vivo.

KW - 3,4,5-TRISPHOSPHATE

KW - HIGH-PRESSURE

KW - ORGANIZATION

KW - INOSITOL TRISPHOSPHATE

KW - IDENTIFICATION

KW - PHOSPHOINOSITIDES

KW - X-RAY

KW - PHASE-TRANSITIONS

KW - DOMAINS

KW - CLATHRIN-DEPENDENT ENDOCYTOSIS

U2 - 10.1039/c2sm07358g

DO - 10.1039/c2sm07358g

M3 - Article (Academic Journal)

SN - 1744-683X

VL - 8

SP - 3090

EP - 3093

JO - Soft Matter

JF - Soft Matter

IS - 11

ER -

Lipid membrane curvature induced by distearoyl phosphatidylinositol 4-phosphate

Abstract

Keywords

Access to Document

Handle.net

Fingerprint

Cite this