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Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis

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Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis. / Gurevich, David B.; French, Kathryn E.; Collin, John D.; Cross, Stephen J.; Martin, Paul.

In: Journal of Cell Science, Vol. 133, 26.09.2019.

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@article{93f5bae515074b5bb7e70fc185bfbd6d,
title = "Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis",
abstract = "Implanting biomaterials in tissues leads to inflammation and a Foreign Body Response (FBR), which can result in rejection. Here we live image the FBR triggered by surgical suture implantation in a translucent zebrafish model and compare with an acute wound response. We observe inflammation extending from the suture margins, and correlating with subsequent avascular and fibrotic encapsulation zones: sutures that induce more inflammation result in increased zones of avascularity and fibrosis. Moreover, we capture macrophages as they fuse to become multinucleate foreign body giant cells (FBGCs) adjacent to the most pro-inflammatory sutures. Genetic and pharmacological dampening of the inflammatory response minimises the FBR (including FBGC generation) and normalises the status of the tissue surrounding these sutures. This model of FBR in adult zebrafish allows us to live image the process and to modulate it in ways that may lead us towards new strategies to ameliorate and circumvent FBR in humans.",
keywords = "Angiogenesis, Fibrosis, Foreign body, Inflammation, Wound, Zebrafish",
author = "Gurevich, {David B.} and French, {Kathryn E.} and Collin, {John D.} and Cross, {Stephen J.} and Paul Martin",
year = "2019",
month = "9",
day = "26",
doi = "10.1242/jcs.236075",
language = "English",
volume = "133",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis

AU - Gurevich, David B.

AU - French, Kathryn E.

AU - Collin, John D.

AU - Cross, Stephen J.

AU - Martin, Paul

PY - 2019/9/26

Y1 - 2019/9/26

N2 - Implanting biomaterials in tissues leads to inflammation and a Foreign Body Response (FBR), which can result in rejection. Here we live image the FBR triggered by surgical suture implantation in a translucent zebrafish model and compare with an acute wound response. We observe inflammation extending from the suture margins, and correlating with subsequent avascular and fibrotic encapsulation zones: sutures that induce more inflammation result in increased zones of avascularity and fibrosis. Moreover, we capture macrophages as they fuse to become multinucleate foreign body giant cells (FBGCs) adjacent to the most pro-inflammatory sutures. Genetic and pharmacological dampening of the inflammatory response minimises the FBR (including FBGC generation) and normalises the status of the tissue surrounding these sutures. This model of FBR in adult zebrafish allows us to live image the process and to modulate it in ways that may lead us towards new strategies to ameliorate and circumvent FBR in humans.

AB - Implanting biomaterials in tissues leads to inflammation and a Foreign Body Response (FBR), which can result in rejection. Here we live image the FBR triggered by surgical suture implantation in a translucent zebrafish model and compare with an acute wound response. We observe inflammation extending from the suture margins, and correlating with subsequent avascular and fibrotic encapsulation zones: sutures that induce more inflammation result in increased zones of avascularity and fibrosis. Moreover, we capture macrophages as they fuse to become multinucleate foreign body giant cells (FBGCs) adjacent to the most pro-inflammatory sutures. Genetic and pharmacological dampening of the inflammatory response minimises the FBR (including FBGC generation) and normalises the status of the tissue surrounding these sutures. This model of FBR in adult zebrafish allows us to live image the process and to modulate it in ways that may lead us towards new strategies to ameliorate and circumvent FBR in humans.

KW - Angiogenesis

KW - Fibrosis

KW - Foreign body

KW - Inflammation

KW - Wound

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85072717262&partnerID=8YFLogxK

U2 - 10.1242/jcs.236075

DO - 10.1242/jcs.236075

M3 - Article

C2 - 31444283

AN - SCOPUS:85072717262

VL - 133

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

ER -