Live vaccine infection burden elicits adaptive humoral and cellular immunity required to prevent Zika virus infection

Clement Yau, Esther Shuyi Gan, Swee Sen Kwek, Hwee Cheng Tan, Eugenia Z Ong, Noor Zayanah Hamis, Laura Rivino, Kuan Rong Chan, Satoru Watanabe, Subhash G Vasudevan, Eng Eong Ooi*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

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BACKGROUND: The emergence of Zika virus (ZIKV) as an important cause of congenital and childhood developmental disorders presents another challenge to global health. Efforts to develop a Zika vaccine have begun although vaccine development against flaviviruses, of which ZIKV belongs to, has proven to be time-consuming and challenging. Defining the vaccine attributes that elicit adaptive immune response necessary for preventing ZIKV infection could provide an evidence-based guide to Zika vaccine development.

METHODS: We used a previously described attenuated ZIKV DN-2 strain in a type-I interferon receptor deficient mouse model and tested the hypothesis that duration of vaccine burden rather than peak level of infection, is a determinant of immunogenicity. We quantified both humoral and cellular responses against ZIKV using plaque reduction neutralisation test and flow cytometry with ELISPOT assays, respectively. Vaccinated mice were challenged with wild-type ZIKV (H/PF/2013 strain) to determine the level of protection against infection.

FINDINGS: We found that the overall vaccine burden is directly correlated with neutralising antibody titres. Reduced duration of vaccine burden lowered neutralising antibody titres that resulted in subclinical infection, despite unchanged peak vaccine viraemia levels. We also found that sterilising immunity is dependant on both neutralising antibody and CD8+T cell responses; depletion of CD8+T cells in vaccinated animals led to wild-type ZIKV infection, especially in the male reproductive tract.

INTERPRETATION: Our findings indicate that duration of attenuated virus vaccine burden is a determinant of humoral and cellular immunity and also suggest that vaccines that elicit both arms of the adaptive immune response are needed to fully prevent ZIKV transmission.

FUNDING: This study was supported by the National Medical Research Council through the Clinician-Scientist Award (Senior Investigator) to E.E.O. Salary support for S.W. was from a Competitive Research Programme grant awarded by the National Research Foundation of Singapore.

Original languageEnglish
Article number103028
Number of pages11
Early online date8 Oct 2020
Publication statusE-pub ahead of print - 8 Oct 2020


  • Vaccine burden
  • Zika virus (ZIKV)
  • Neutralizing antibodies
  • CD8+ T cells
  • Sterilizing immunity


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