Local and plasma antibody responses to the parasitic larval stages of the abomasal nematode Ostertagia circumcincta

E Sinski, K Bairden, JL Duncan, MC Eisler, PH Holmes, QA McKellar, M Murray, MJ Stear

    Research output: Contribution to journalArticle (Academic Journal)peer-review

    50 Citations (Scopus)

    Abstract

    Ovine isotype-specific antibody responses to the parasitic larval stages of the abomasal nematode Ostertagia circumcincta were measured in a simple, indirect enzyme-linked immunosorbent assay. Analysis of variance of replicate tests showed that the assay was very reliable. There was substantial variation among individual sheep in their IgA and IgG1 responses even though the sheep had been matched for breed, age and sex, were born on the same farm, were reared identically and had the same history of exposure and challenge with O. circumcincta. The local IgA responses to a somatic extract of fourth-stage larvae were very similar to responses to excretory-secretory products of fourth-stage larvae. The responses to third stage larvae were correlated with the responses to fourth stage larvae. There was a negative correlation between parasite-specific plasma IgG1 and parasite-specific plasma IgA responses. There was only a moderate association between IgA responses in the mucus and the plasma. Therefore, antibody responses measured in plasma cannot be easily extrapolated to antibody responses in the abomasal mucus.

    Translated title of the contributionLocal and plasma antibody responses to the parasitic larval stages of the abomasal nematode Ostertagia circumcincta
    Original languageEnglish
    Pages (from-to)107-118
    Number of pages12
    JournalVeterinary Parasitology
    Volume59
    Issue number2
    Publication statusPublished - Sep 1995

    Keywords

    • SHEEP-NEMATODA
    • OSTERTAGIA CIRCUMCINCTA
    • TELADORSAGIA CIRCUMCINCTA
    • IMMUNITY-NEMATODA
    • T-CELL CLONE
    • IMMUNE-RESPONSES
    • PROTEINS
    • LAMBS
    • SUBSETS

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