TY - JOUR
T1 - Longitudinal high-resolution imaging through a flexible intravital imaging window
AU - Jacquemin, Guillaume
AU - Benavente-Diaz, Maria
AU - Djaber, Samir
AU - Bore, Aurelien
AU - Dangles-Marie, Virginie
AU - Surdez, Didier
AU - Tajbakhsh, Shahragim
AU - Fre, Silvia
AU - Lloyd-Lewis, Bethan
PY - 2021/6/16
Y1 - 2021/6/16
N2 - Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, suture-less design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening new avenues for in vivo imaging of soft and fragile tissues
AB - Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, suture-less design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening new avenues for in vivo imaging of soft and fragile tissues
M3 - Article (Academic Journal)
JO - Science Advances
JF - Science Advances
SN - 2375-2548
ER -