Longitudinal high-resolution imaging through a flexible intravital imaging window

Guillaume Jacquemin*, Maria Benavente-Diaz, Samir Djaber, Aurelien Bore, Virginie Dangles-Marie, Didier Surdez, Shahragim Tajbakhsh, Silvia Fre*, Bethan Lloyd-Lewis*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Abstract

Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, suture-less design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening new avenues for in vivo imaging of soft and fragile tissues
Original languageEnglish
Number of pages29
JournalScience Advances
Publication statusPublished - 16 Jun 2021

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