Loss of myosin VI expression affects acrosome/acroplaxome complex morphology during mouse spermiogenesis

Przemyslaw Zakrzewski; Maria Jolanta Redowicz; Folma Buss; Marta Lenartowska

doi:10.1093/biolre/ioaa071

Loss of myosin VI expression affects acrosome/acroplaxome complex morphology during mouse spermiogenesis

Przemyslaw Zakrzewski^*
, Maria Jolanta Redowicz
, Folma Buss
, Marta Lenartowska

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

23 Citations (Scopus)

Abstract

During spermiogenesis in mammals, actin filaments and a variety of actin-binding proteins are involved in the formation and function of highly specialized testis-specific structures. Actin-based motor proteins, such as myosin Va and VIIa, play a key role in this complex process of spermatid transformation into mature sperm. We have previously demonstrated that myosin VI (MYO6) is also expressed in mouse testes. It is present in actin-rich structures important for spermatid development, including one of the earliest events in spermiogenesis-acrosome formation. Here, we demonstrate using immunofluorescence, cytochemical, and ultrastructural approaches that MYO6 is involved in maintaining the structural integrity of these specialized actin-rich structures during acrosome biogenesis in mouse. We show that MYO6 together with its binding partner TOM1/L2 is present at/around the spermatid Golgi complex and the nascent acrosome. Depletion of MYO6 in Snell's waltzer mice causes structural disruptions of the Golgi complex and affects the acrosomal granule positioning within the developing acrosome. In summary, our results suggest that MYO6 plays an anchoring role during the acrosome biogenesis mainly by tethering of different cargo/membranes to highly specialized actin-related structures.

Original language	English
Pages (from-to)	521-533
Number of pages	13
Journal	Biology of Reproduction
Volume	103
Issue number	3
DOIs	https://doi.org/10.1093/biolre/ioaa071
Publication status	Published - 21 Aug 2020

Bibliographical note

Funding Information:
*Correspondence: Department of Cellular and Molecular Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Torun, Poland. Tel: +48 56 611 31 86; E-mail: [email protected] †Grant Support: This project was supported by PRELUDIUM grant from the National Science Centre (Poland) to P.Z. (2017/25/N/NZ3/00487), an ETIUDA doctoral scholarship from the National Science Centre (Poland) to P.Z. (2018/28/T/NZ3/00002), a traveling fellowship for P.Z. funded by The Company of Biologists (JCSTF-171105) and a Medical Research Council program grant to F.B. (MR/S007776/1). Conference Presentation: Presented in part at the 43rd FEBS Congress, 7–12 July 2018, Prague, Czech Republic, and the 44th FEBS Congress, 6–11 July 2019, Krakow, Poland. F.B. and M.L. contributed equally to this work and are joint senior authors.

Publisher Copyright:
© The Author(s) 2020. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved.

Keywords

Acroplaxome
Acrosome/cap
Actin cytoskeleton
Golgi complex
Myosin VI
Snell's waltzer mice

Access to Document

10.1093/biolre/ioaa071

Handle.net

Persistent link

Cite this

@article{1754cb70f16c40bbb7202c60fca4aa9a,

title = "Loss of myosin VI expression affects acrosome/acroplaxome complex morphology during mouse spermiogenesis",

abstract = "During spermiogenesis in mammals, actin filaments and a variety of actin-binding proteins are involved in the formation and function of highly specialized testis-specific structures. Actin-based motor proteins, such as myosin Va and VIIa, play a key role in this complex process of spermatid transformation into mature sperm. We have previously demonstrated that myosin VI (MYO6) is also expressed in mouse testes. It is present in actin-rich structures important for spermatid development, including one of the earliest events in spermiogenesis-acrosome formation. Here, we demonstrate using immunofluorescence, cytochemical, and ultrastructural approaches that MYO6 is involved in maintaining the structural integrity of these specialized actin-rich structures during acrosome biogenesis in mouse. We show that MYO6 together with its binding partner TOM1/L2 is present at/around the spermatid Golgi complex and the nascent acrosome. Depletion of MYO6 in Snell's waltzer mice causes structural disruptions of the Golgi complex and affects the acrosomal granule positioning within the developing acrosome. In summary, our results suggest that MYO6 plays an anchoring role during the acrosome biogenesis mainly by tethering of different cargo/membranes to highly specialized actin-related structures.",

keywords = "Acroplaxome, Acrosome/cap, Actin cytoskeleton, Golgi complex, Myosin VI, Snell's waltzer mice",

author = "Przemyslaw Zakrzewski and Redowicz, \{Maria Jolanta\} and Folma Buss and Marta Lenartowska",

note = "Funding Information: *Correspondence: Department of Cellular and Molecular Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toru{\'n}, Lwowska 1, 87-100 Torun, Poland. Tel: +48 56 611 31 86; E-mail: [email protected] †Grant Support: This project was supported by PRELUDIUM grant from the National Science Centre (Poland) to P.Z. (2017/25/N/NZ3/00487), an ETIUDA doctoral scholarship from the National Science Centre (Poland) to P.Z. (2018/28/T/NZ3/00002), a traveling fellowship for P.Z. funded by The Company of Biologists (JCSTF-171105) and a Medical Research Council program grant to F.B. (MR/S007776/1). Conference Presentation: Presented in part at the 43rd FEBS Congress, 7–12 July 2018, Prague, Czech Republic, and the 44th FEBS Congress, 6–11 July 2019, Krakow, Poland. F.B. and M.L. contributed equally to this work and are joint senior authors. Publisher Copyright: {\textcopyright} The Author(s) 2020. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved.",

year = "2020",

month = aug,

day = "21",

doi = "10.1093/biolre/ioaa071",

language = "English",

volume = "103",

pages = "521--533",

journal = "Biology of Reproduction",

issn = "0006-3363",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Loss of myosin VI expression affects acrosome/acroplaxome complex morphology during mouse spermiogenesis

AU - Zakrzewski, Przemyslaw

AU - Redowicz, Maria Jolanta

AU - Buss, Folma

AU - Lenartowska, Marta

N1 - Funding Information: *Correspondence: Department of Cellular and Molecular Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Torun, Poland. Tel: +48 56 611 31 86; E-mail: [email protected] †Grant Support: This project was supported by PRELUDIUM grant from the National Science Centre (Poland) to P.Z. (2017/25/N/NZ3/00487), an ETIUDA doctoral scholarship from the National Science Centre (Poland) to P.Z. (2018/28/T/NZ3/00002), a traveling fellowship for P.Z. funded by The Company of Biologists (JCSTF-171105) and a Medical Research Council program grant to F.B. (MR/S007776/1). Conference Presentation: Presented in part at the 43rd FEBS Congress, 7–12 July 2018, Prague, Czech Republic, and the 44th FEBS Congress, 6–11 July 2019, Krakow, Poland. F.B. and M.L. contributed equally to this work and are joint senior authors. Publisher Copyright: © The Author(s) 2020. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved.

PY - 2020/8/21

Y1 - 2020/8/21

N2 - During spermiogenesis in mammals, actin filaments and a variety of actin-binding proteins are involved in the formation and function of highly specialized testis-specific structures. Actin-based motor proteins, such as myosin Va and VIIa, play a key role in this complex process of spermatid transformation into mature sperm. We have previously demonstrated that myosin VI (MYO6) is also expressed in mouse testes. It is present in actin-rich structures important for spermatid development, including one of the earliest events in spermiogenesis-acrosome formation. Here, we demonstrate using immunofluorescence, cytochemical, and ultrastructural approaches that MYO6 is involved in maintaining the structural integrity of these specialized actin-rich structures during acrosome biogenesis in mouse. We show that MYO6 together with its binding partner TOM1/L2 is present at/around the spermatid Golgi complex and the nascent acrosome. Depletion of MYO6 in Snell's waltzer mice causes structural disruptions of the Golgi complex and affects the acrosomal granule positioning within the developing acrosome. In summary, our results suggest that MYO6 plays an anchoring role during the acrosome biogenesis mainly by tethering of different cargo/membranes to highly specialized actin-related structures.

AB - During spermiogenesis in mammals, actin filaments and a variety of actin-binding proteins are involved in the formation and function of highly specialized testis-specific structures. Actin-based motor proteins, such as myosin Va and VIIa, play a key role in this complex process of spermatid transformation into mature sperm. We have previously demonstrated that myosin VI (MYO6) is also expressed in mouse testes. It is present in actin-rich structures important for spermatid development, including one of the earliest events in spermiogenesis-acrosome formation. Here, we demonstrate using immunofluorescence, cytochemical, and ultrastructural approaches that MYO6 is involved in maintaining the structural integrity of these specialized actin-rich structures during acrosome biogenesis in mouse. We show that MYO6 together with its binding partner TOM1/L2 is present at/around the spermatid Golgi complex and the nascent acrosome. Depletion of MYO6 in Snell's waltzer mice causes structural disruptions of the Golgi complex and affects the acrosomal granule positioning within the developing acrosome. In summary, our results suggest that MYO6 plays an anchoring role during the acrosome biogenesis mainly by tethering of different cargo/membranes to highly specialized actin-related structures.

KW - Acroplaxome

KW - Acrosome/cap

KW - Actin cytoskeleton

KW - Golgi complex

KW - Myosin VI

KW - Snell's waltzer mice

UR - https://www.scopus.com/pages/publications/85089794005

U2 - 10.1093/biolre/ioaa071

DO - 10.1093/biolre/ioaa071

M3 - Article (Academic Journal)

C2 - 32412041

AN - SCOPUS:85089794005

SN - 0006-3363

VL - 103

SP - 521

EP - 533

JO - Biology of Reproduction

JF - Biology of Reproduction

IS - 3

ER -

Loss of myosin VI expression affects acrosome/acroplaxome complex morphology during mouse spermiogenesis

Abstract

Bibliographical note

Keywords

Access to Document

Handle.net

Other files and links

Fingerprint

Cite this