Low fossilisation potential of keratin protein revealed by experimental taphonomy

Evan Saitta, Chris S Rogers, Richard Brooker, Geoffrey D Abbott, Sumit Kumar, Shane O'Reilly, Paul Donohoe, Suryendu Dutta, Roger E. Summons, Jakob Vinther

Research output: Contribution to journalArticle (Academic Journal)

32 Citations (Scopus)
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Abstract

Recent studies have suggested the presence of keratin in Cenozoic- to Mesozoic-aged fossils. However, ultrastructural studies revealing exposed melanosomes in many fossil keratinous tissues suggest that keratin should rarely, if ever, be preserved. In this study, keratin’s stability through diagenesis was tested using microbial decay and maturation experiments on various keratinous structures. The residues were analyzed by pyrolysis-gas chromatography-mass spectrometry and compared to unpublished feather and hair fossils and published fresh and fossil melanin from squid ink. Results show that highly matured feathers (200–250 °C/250 bars/24 hours) become a volatile-rich, thick fluid with semi-distinct pyrolysis compounds from those observed in less degraded keratins (i.e., fresh, decayed, moderately matured, and decayed and moderately matured), suggesting hydrolysis of peptide bonds and potential degradation of free amino acids. Neither melanization nor keratin (secondary) structure (e.g., ⍺- vs. β-keratin) produced different pyrograms – melanin pyrolysates are largely a subset of those from proteins and proteins have characteristic pyrolysates. Analyses of fossil fur and feather lacked amides, succinimide, and piperazines (present even in highly matured keratin) and showed pyrolysis compounds more similar to fossil and fresh melanin than to non-matured or matured keratin. Although the highly matured fluid was not water soluble at room temperature, it readily dissolved at elevated temperatures easily attained during diagenesis, meaning it can leach away from the fossil. Future interpretations of fossils must consider that calcium phosphate and pigments are the only components of keratinous structures known to survive fossilisation in mature sediments.
Original languageEnglish
Pages (from-to)547-556
Number of pages10
JournalPalaeontology
Volume60
Issue number4
Early online date8 May 2017
DOIs
Publication statusPublished - Jul 2017

Keywords

  • keratin
  • protein
  • experimental taphonomy
  • Py-GC-MS
  • fossilisation

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    Saitta, E., Rogers, C. S., Brooker, R., Abbott, G. D., Kumar, S., O'Reilly, S., Donohoe, P., Dutta, S., Summons, R. E., & Vinther, J. (2017). Low fossilisation potential of keratin protein revealed by experimental taphonomy. Palaeontology, 60(4), 547-556. https://doi.org/10.1111/pala.12299