TY - JOUR
T1 - Low-resolution structure determination of Na(+)-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae by ab initio phasing and electron microscopy
AU - Lunin, Vladimir Y
AU - Lunina, Natalia L
AU - Casutt, Marco S
AU - Knoops, Kèvin
AU - Schaffitzel, Christiane
AU - Steuber, Julia
AU - Fritz, Günter
AU - Baumstark, Manfred W
PY - 2012/6
Y1 - 2012/6
N2 - A low-resolution structure of the Na(+)-translocating NADH:ubiquinone oxidoreductase from the human pathogen Vibrio cholerae was determined by ab initio phasing and independently confirmed by electron microscopy. This multi-subunit membrane-protein complex (molecular weight 210 kDa) generates an Na(+) gradient that is essential for substrate uptake, motility, pathogenicity and efflux of antibiotics. The obtained 16 Å resolution electron density-map revealed an asymmetric particle with a central region of low electron density and a putative detergent region, and allowed the identification of the transmembrane regions of the complex.
AB - A low-resolution structure of the Na(+)-translocating NADH:ubiquinone oxidoreductase from the human pathogen Vibrio cholerae was determined by ab initio phasing and independently confirmed by electron microscopy. This multi-subunit membrane-protein complex (molecular weight 210 kDa) generates an Na(+) gradient that is essential for substrate uptake, motility, pathogenicity and efflux of antibiotics. The obtained 16 Å resolution electron density-map revealed an asymmetric particle with a central region of low electron density and a putative detergent region, and allowed the identification of the transmembrane regions of the complex.
KW - Computational Biology
KW - Electron Transport Complex I
KW - Microscopy, Electron
KW - Models, Molecular
KW - Protein Structure, Tertiary
KW - Structural Homology, Protein
KW - Vibrio cholerae
U2 - 10.1107/S0907444912012012
DO - 10.1107/S0907444912012012
M3 - Article (Academic Journal)
C2 - 22683795
SN - 0907-4449
VL - 68
SP - 724
EP - 731
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 6
ER -