Abstract
Chloroplasts are a common feature of plant cells and aspects of their metabolism, including photosynthesis, are influenced by low-temperature conditions. Chloroplasts contain a small circular genome that encodes essential components of the photosynthetic apparatus and chloroplast transcription/translation machinery. Here, we show that in Arabidopsis, a nuclear-encoded sigma factor that controls chloroplast transcription (SIGMA FACTOR5) contributes to adaptation to low-temperature conditions. This process involves the regulation of SIGMA FACTOR5 expression in response to cold by the bZIP transcription factors ELONGATED HYPOCOTYL5 and ELONGATED HYPOCOTYL5 HOMOLOG. The response of this pathway to cold is gated by the circadian clock, and it enhances photosynthetic efficiency during long-term cold and freezing exposure. We identify a process that integrates low-temperature and circadian signals, and modulates the response of chloroplasts to low-temperature conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 661–672 |
| Number of pages | 12 |
| Journal | Nature Plants |
| Volume | 9 |
| Issue number | 4 |
| Early online date | 30 Mar 2023 |
| DOIs | |
| Publication status | Published - 1 Apr 2023 |
Bibliographical note
Funding Information:This research was funded by Biotechnology & Biological Sciences Research Council (UK) (BB/I005811/2, BB/J014400/1, BB/T003030/1, Institute Strategic Programme GEN BB/P013511/1 to A.N.D.; studentship 1518540 awarded to P.E.P.), Norwich Research Park Doctoral Training Partnership (BB/T008717/1, to R.D. and A.B.), The Leverhulme Trust (RPG-2018-216, to A.N.D.), the Bristol Centre for Agricultural Innovation (to A.N.D.), the Wolfson Foundation (to A.N.D.), NAGASE Science Technology Foundation (Japan), the Ministry of Education, Culture, Sports, Science and Technology (Japan) (Grants-in-Aid 17K07438, to K.T. and S.I.) and Tokyo Institute of Technology World Research Hub Initiative Program of Institute of Innovative Research (to K.T.). D.L.C.-R. is grateful to the Consejo Nacional de Ciencia y Tecnología (Mexico) for granting a PhD scholarship. We thank the University of Bristol Genomics Facility and Z. Song for experimental support; G. Jenkins for seed donation; S. Samwald, E. Tee, J. Sallmen and N. Holmes for help with protein analysis; C. Faulkner, T. Oyama and T. Muranaka for advice about transient expression; and M. Knight, Y. Yoshitake and M. Shimojima for technical advice. Figure 5 created with BioRender.com .
Funding Information:
This research was funded by Biotechnology & Biological Sciences Research Council (UK) (BB/I005811/2, BB/J014400/1, BB/T003030/1, Institute Strategic Programme GEN BB/P013511/1 to A.N.D.; studentship 1518540 awarded to P.E.P.), Norwich Research Park Doctoral Training Partnership (BB/T008717/1, to R.D. and A.B.), The Leverhulme Trust (RPG-2018-216, to A.N.D.), the Bristol Centre for Agricultural Innovation (to A.N.D.), the Wolfson Foundation (to A.N.D.), NAGASE Science Technology Foundation (Japan), the Ministry of Education, Culture, Sports, Science and Technology (Japan) (Grants-in-Aid 17K07438, to K.T. and S.I.) and Tokyo Institute of Technology World Research Hub Initiative Program of Institute of Innovative Research (to K.T.). D.L.C.-R. is grateful to the Consejo Nacional de Ciencia y Tecnología (Mexico) for granting a PhD scholarship. We thank the University of Bristol Genomics Facility and Z. Song for experimental support; G. Jenkins for seed donation; S. Samwald, E. Tee, J. Sallmen and N. Holmes for help with protein analysis; C. Faulkner, T. Oyama and T. Muranaka for advice about transient expression; and M. Knight, Y. Yoshitake and M. Shimojima for technical advice. Figure 5 created with BioRender.com.
Publisher Copyright:
© 2023, The Author(s).
Keywords
- Plants
- cold
- circadian clock
- sigma factor