LuxS-based quorum sensing does not affect the ability of Salmonella enterica serovar Typhimurium to express the SPI-1 type 3 secretion system, induce membrane ruffles, or invade epithelial cells

CA Perrett, MH Karavolos, S Humphrey, P Mastroeni, I Martinez-Argudo, H Spencer, D Bulmer, K Winzer, E McGhie, V Koronakis, P Williams, CMA Khan, MA Jepson

Research output: Contribution to journalArticle (Academic Journal)

19 Citations (Scopus)

Abstract

Bacterial species can communicate by producing and sensing small autoinducer molecules by a process known as quorum sensing. Salmonella enterica produces autoinducer 2 (AI-2) via the luxS synthase gene, which is used by some bacterial pathogens to coordinate virulence gene expression with population density. We investigated whether the luxS gene might affect the ability of Salmonella enterica serovar Typhimurium to invade epithelial cells. No differences were found between the wild-type strain of S. Typhimurium, SL1344, and its isogenic luxS mutant with respect to the number and morphology of the membrane ruffles induced or their ability to invade epithelial cells. The dynamics of the ruffling process were also similar in the wild-type strain (SL1344) and the luxS mutant. Furthermore, comparing the Salmonella pathogenicity island 1 (SPI-1) type 3 secretion profiles of wild-type SL1344 and the luxS mutant by Western blotting and measuring the expression of a single-copy green fluorescent protein fusion to the prgH (an essential SPI-1 gene) promoter indicated that SPI-1 expression and activity are similar in the wild-type SL1344 and luxS mutant. Genetic deletion of luxS did not alter the virulence of S.Typhimurium in the mouse model, and therefore, it appears that luxS does not play a significant role in regulating invasion of Salmonella in vitro or in vivo.
Translated title of the contributionLuxS-based quorum sensing does not affect the ability of Salmonella enterica serovar Typhimurium to express the SPI-1 type 3 secretion system, induce membrane ruffles, or invade epithelial cells
Original languageEnglish
Pages (from-to)7253 - 7259
Number of pages7
JournalJournal of Bacteriology
Volume191 (23)
DOIs
Publication statusPublished - Dec 2009

Fingerprint Dive into the research topics of 'LuxS-based quorum sensing does not affect the ability of <i>Salmonella enterica</i> serovar Typhimurium to express the SPI-1 type 3 secretion system, induce membrane ruffles, or invade epithelial cells'. Together they form a unique fingerprint.

  • Cite this

    Perrett, CA., Karavolos, MH., Humphrey, S., Mastroeni, P., Martinez-Argudo, I., Spencer, H., Bulmer, D., Winzer, K., McGhie, E., Koronakis, V., Williams, P., Khan, CMA., & Jepson, MA. (2009). LuxS-based quorum sensing does not affect the ability of Salmonella enterica serovar Typhimurium to express the SPI-1 type 3 secretion system, induce membrane ruffles, or invade epithelial cells. Journal of Bacteriology, 191 (23), 7253 - 7259. https://doi.org/10.1128/JB.00727-09