Maintenance of hypertensive hemodynamics does not depend on ROS in established experimental chronic kidney disease

Diana A Papazova, Arianne van Koppen, Maarten P Koeners, Ronald L Bleys, Marianne C Verhaar, Jaap A Joles

Research output: Contribution to journalArticle (Academic Journal)

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Abstract

While the presence of oxidative stress in chronic kidney disease (CKD) is well established, its relation to hypertensive renal hemodynamics remains unclear. We hypothesized that once CKD is established blood pressure and renal vascular resistance (RVR) no longer depend on reactive oxygen species. CKD was induced by bilateral ablation of 2/3 of each kidney. Compared to age-matched, sham-operated controls all ablated rats showed proteinuria, decreased glomerular filtration rate (GFR), more renal damage, higher mean arterial pressure (MAP), RVR and excretion of oxidative stress markers and hydrogen peroxide, while excretion of stable nitric oxide (NO) metabolites tended to decrease. We compared MAP, RVR, GFR and fractional excretion of sodium under baseline and during acute Tempol, PEG-catalase or vehicle infusion in rats with established CKD vs. controls. Tempol caused marked reduction in MAP in controls (96±5 vs.79±4 mmHg, P<0.05) but not in CKD (130±5 vs. 127±6 mmHg). PEG-catalase reduced MAP in both groups (controls: 102±2 vs. 94±4 mmHg, P<0.05; CKD: 118±4 vs. 110±4 mmHg, P<0.05), but did not normalize MAP in CKD rats. Tempol and PEG-catalase slightly decreased RVR in both groups. Fractional excretion of sodium was increased by both Tempol and PEG-catalase in both groups. PEG-catalase decreased TBARS excretion in both groups. In sum, although oxidative stress markers were increased, MAP and RVR did not depend more on oxidative stress in CKD than in controls. Therefore reactive oxygen species appear not to be important direct determinants of hypertensive renal hemodynamics in this model of established CKD.

Original languageEnglish
Pages (from-to)e88596
JournalPLoS ONE
Volume9
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • Animals
  • Blood Pressure
  • Catalase
  • Cyclic N-Oxides
  • Gene Expression Profiling
  • Glomerular Filtration Rate
  • Hemodynamics
  • Hydrogen Peroxide
  • Hypertension
  • Kidney
  • Kidney Failure, Chronic
  • Male
  • Organ Size
  • Oxidative Stress
  • Peptidyl-Dipeptidase A
  • Polyethylene Glycols
  • Rats
  • Rats, Inbred Lew
  • Reactive Oxygen Species
  • Renin
  • Spin Labels
  • Thiobarbituric Acid Reactive Substances
  • Time Factors
  • Vascular Endothelial Growth Factor A

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    Papazova, D. A., van Koppen, A., Koeners, M. P., Bleys, R. L., Verhaar, M. C., & Joles, J. A. (2014). Maintenance of hypertensive hemodynamics does not depend on ROS in established experimental chronic kidney disease. PLoS ONE, 9(2), e88596. https://doi.org/10.1371/journal.pone.0088596