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Maternal BMI at the start of pregnancy and offspring epigenome-wide DNA methylation: findings from the Pregnancy and Childhood Epigenetics (PACE) consortium

Research output: Contribution to journalArticle (Academic Journal)

Original languageEnglish
Pages (from-to)4067-4085
Number of pages19
JournalHuman Molecular Genetics
Volume26
Issue number20
Early online date21 Jul 2017
DOIs
DateAccepted/In press - 17 Jul 2017
DateE-pub ahead of print - 21 Jul 2017
DatePublished (current) - 15 Oct 2017

Abstract

Pre-pregnancy maternal obesity is associated with adverse offspring outcomes at birth and later in life. Individual studies have shown that epigenetic modifications such as DNA methylation could contribute. Within the Pregnancy and Childhood Epigenetics (PACE) Consortium, we meta-analysed the association between pre-pregnancy maternal BMI and methylation at over 450,000 sites in newborn blood DNA, across 19 cohorts (9,340 mother-newborn pairs). We attempted to infer causality by comparing effects of maternal versus paternal BMI and incorporating genetic variation. In four additional cohorts (1,817 mother-child pairs), we meta-analysed the association between maternal BMI at the start of pregnancy and blood methylation in adolescents. In newborns, maternal BMI was associated with small (<0.2% per BMI unit (1kg/m2), P<1.06*10-7) methylation variation at 9,044 sites throughout the genome. Adjustment for estimated cell proportions greatly attenuated the number of significant CpGs to 104, including 86 sites common to the unadjusted model. At 72/86 sites, the direction of association was the same in newborns and adolescents, suggesting persistence of signals. However, we found evidence for a causal intrauterine effect of maternal BMI on newborn methylation at just 8/86 sites. In conclusion, this well-powered analysis identified robust associations between maternal adiposity and variations in newborn blood DNA methylation, but these small effects may be better explained by genetic or lifestyle factors than a causal intrauterine mechanism. This highlights the need for large-scale collaborative approaches and the application of causal inference techniques in epigenetic epidemiology.

    Research areas

  • Body Mass Index, BMI, Adiposity, Epigenetics, Negative control, Causal inference, Prenatal, Epidemiology, Pregnancy

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Oxford University Press at https://academic.oup.com/hmg/article/doi/10.1093/hmg/ddx290/3988352/Maternal-BMI-at-the-start-of-pregnancy-and. Please refer to any applicable terms of use of the publisher.

    Final published version, 1.09 MB, PDF document

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Oxford University Press at https://academic.oup.com/hmg/article/doi/10.1093/hmg/ddx290/3988352/Maternal-BMI-at-the-start-of-pregnancy-and. Please refer to any applicable terms of use of the publisher.

    Final published version, 2.59 MB, PDF document

    Licence: CC BY

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