Maternal Dietary Glycemic Index and Glycemic Load in Pregnancy and Offspring Cord Blood DNA Methylation

Leanne K Küpers; Sílvia Fernández-Barrés; Giulia Mancano; Laura Johnson; Raffael Ott; Jesus Vioque; Marco Colombo; Kathrin Landgraf; Elmar W Tobi; Antje Körner; Romy Gaillard; Jeanne H M de Vries; Vincent W V Jaddoe; Martine Vrijheid; Gemma C Sharp; Janine F Felix

doi:10.2337/dc21-2662

Maternal Dietary Glycemic Index and Glycemic Load in Pregnancy and Offspring Cord Blood DNA Methylation

Leanne K Küpers, Sílvia Fernández-Barrés, Giulia Mancano, Laura Johnson, Raffael Ott, Jesus Vioque, Marco Colombo, Kathrin Landgraf, Elmar W Tobi, Antje Körner, Romy Gaillard, Jeanne H M de Vries, Vincent W V Jaddoe, Martine Vrijheid, Gemma C Sharp, Janine F Felix^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

12 Citations (Scopus)

Abstract

OBJECTIVE: Suboptimal nutrition in pregnancy is associated with worse offspring cardiometabolic health. DNA methylation may be an underlying mechanism. We meta-analyzed epigenome-wide association studies (EWAS) of maternal dietary glycemic index and load with cord blood DNA methylation.

RESEARCH DESIGN AND METHODS: We calculated maternal glycemic index and load from food frequency questionnaires and ran EWAS on cord blood DNA methylation in 2,003 mother-offspring pairs from three cohorts. Analyses were additionally stratified by maternal BMI categories. We looked-up the findings in EWAS of maternal glycemic traits and BMI as well as in EWAS of birth weight and child BMI. We examined associations with gene expression in child blood in the online Human Early Life Exposome eQTM catalog and in 223 adipose tissue samples.

RESULTS: Maternal glycemic index and load were associated with cord blood DNA methylation at 41 cytosine-phosphate-guanine sites (CpGs, P < 1.17 × 10-7), mostly in mothers with overweight/obesity. We did not observe overlap with CpGs associated with maternal glycemic traits, BMI, or child birth weight or BMI. Only DNA methylation at cg24458009 and cg23347399 was associated with expression of PCED1B and PCDHG, respectively, in child blood, and DNA methylation at cg27193519 was associated with expression of TFAP4, ZNF500, PPL, and ANKS3 in child subcutaneous adipose tissue.

CONCLUSIONS: We observed multiple associations of maternal glycemic index and load during pregnancy with cord blood DNA methylation, mostly in mothers with overweight/obesity; some of these CpGs were associated with gene expression. Additional studies are required to further explore functionality, uncover causality, and study pathways to offspring health.

Original language	English
Pages (from-to)	1822-1832
Number of pages	11
Journal	Diabetes Care
Volume	45
Issue number	8
Early online date	16 Jun 2022
DOIs	https://doi.org/10.2337/dc21-2662
Publication status	E-pub ahead of print - 16 Jun 2022

Bibliographical note

Publisher Copyright:
© 2022 by the American Diabetes Association.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.2337/dc21-2662

Handle.net

Persistent link

Cite this

Küpers, L. K., Fernández-Barrés, S., Mancano, G., Johnson, L., Ott, R., Vioque, J., Colombo, M., Landgraf, K., Tobi, E. W., Körner, A., Gaillard, R., de Vries, J. H. M., Jaddoe, V. W. V., Vrijheid, M., Sharp, G. C., & Felix, J. F. (2022). Maternal Dietary Glycemic Index and Glycemic Load in Pregnancy and Offspring Cord Blood DNA Methylation. Diabetes Care, 45(8), 1822-1832. Advance online publication. https://doi.org/10.2337/dc21-2662

@article{4c15ce49b6304cd7a1b19d37111d2ad3,

title = "Maternal Dietary Glycemic Index and Glycemic Load in Pregnancy and Offspring Cord Blood DNA Methylation",

abstract = "OBJECTIVE: Suboptimal nutrition in pregnancy is associated with worse offspring cardiometabolic health. DNA methylation may be an underlying mechanism. We meta-analyzed epigenome-wide association studies (EWAS) of maternal dietary glycemic index and load with cord blood DNA methylation.RESEARCH DESIGN AND METHODS: We calculated maternal glycemic index and load from food frequency questionnaires and ran EWAS on cord blood DNA methylation in 2,003 mother-offspring pairs from three cohorts. Analyses were additionally stratified by maternal BMI categories. We looked-up the findings in EWAS of maternal glycemic traits and BMI as well as in EWAS of birth weight and child BMI. We examined associations with gene expression in child blood in the online Human Early Life Exposome eQTM catalog and in 223 adipose tissue samples.RESULTS: Maternal glycemic index and load were associated with cord blood DNA methylation at 41 cytosine-phosphate-guanine sites (CpGs, P < 1.17 × 10-7), mostly in mothers with overweight/obesity. We did not observe overlap with CpGs associated with maternal glycemic traits, BMI, or child birth weight or BMI. Only DNA methylation at cg24458009 and cg23347399 was associated with expression of PCED1B and PCDHG, respectively, in child blood, and DNA methylation at cg27193519 was associated with expression of TFAP4, ZNF500, PPL, and ANKS3 in child subcutaneous adipose tissue.CONCLUSIONS: We observed multiple associations of maternal glycemic index and load during pregnancy with cord blood DNA methylation, mostly in mothers with overweight/obesity; some of these CpGs were associated with gene expression. Additional studies are required to further explore functionality, uncover causality, and study pathways to offspring health.",

author = "K{\"u}pers, {Leanne K} and S{\'i}lvia Fern{\'a}ndez-Barr{\'e}s and Giulia Mancano and Laura Johnson and Raffael Ott and Jesus Vioque and Marco Colombo and Kathrin Landgraf and Tobi, {Elmar W} and Antje K{\"o}rner and Romy Gaillard and {de Vries}, {Jeanne H M} and Jaddoe, {Vincent W V} and Martine Vrijheid and Sharp, {Gemma C} and Felix, {Janine F}",

note = "Publisher Copyright: {\textcopyright} 2022 by the American Diabetes Association.",

year = "2022",

month = jun,

day = "16",

doi = "10.2337/dc21-2662",

language = "English",

volume = "45",

pages = "1822--1832",

journal = "Diabetes Care",

issn = "0149-5992",

publisher = "American Diabetes Association Inc.",

number = "8",

}

Küpers, LK, Fernández-Barrés, S, Mancano, G, Johnson, L, Ott, R, Vioque, J, Colombo, M, Landgraf, K, Tobi, EW, Körner, A, Gaillard, R, de Vries, JHM, Jaddoe, VWV, Vrijheid, M, Sharp, GC & Felix, JF 2022, 'Maternal Dietary Glycemic Index and Glycemic Load in Pregnancy and Offspring Cord Blood DNA Methylation', Diabetes Care, vol. 45, no. 8, pp. 1822-1832. https://doi.org/10.2337/dc21-2662

TY - JOUR

T1 - Maternal Dietary Glycemic Index and Glycemic Load in Pregnancy and Offspring Cord Blood DNA Methylation

AU - Küpers, Leanne K

AU - Fernández-Barrés, Sílvia

AU - Mancano, Giulia

AU - Johnson, Laura

AU - Ott, Raffael

AU - Vioque, Jesus

AU - Colombo, Marco

AU - Landgraf, Kathrin

AU - Tobi, Elmar W

AU - Körner, Antje

AU - Gaillard, Romy

AU - de Vries, Jeanne H M

AU - Jaddoe, Vincent W V

AU - Vrijheid, Martine

AU - Sharp, Gemma C

AU - Felix, Janine F

PY - 2022/6/16

Y1 - 2022/6/16

N2 - OBJECTIVE: Suboptimal nutrition in pregnancy is associated with worse offspring cardiometabolic health. DNA methylation may be an underlying mechanism. We meta-analyzed epigenome-wide association studies (EWAS) of maternal dietary glycemic index and load with cord blood DNA methylation.RESEARCH DESIGN AND METHODS: We calculated maternal glycemic index and load from food frequency questionnaires and ran EWAS on cord blood DNA methylation in 2,003 mother-offspring pairs from three cohorts. Analyses were additionally stratified by maternal BMI categories. We looked-up the findings in EWAS of maternal glycemic traits and BMI as well as in EWAS of birth weight and child BMI. We examined associations with gene expression in child blood in the online Human Early Life Exposome eQTM catalog and in 223 adipose tissue samples.RESULTS: Maternal glycemic index and load were associated with cord blood DNA methylation at 41 cytosine-phosphate-guanine sites (CpGs, P < 1.17 × 10-7), mostly in mothers with overweight/obesity. We did not observe overlap with CpGs associated with maternal glycemic traits, BMI, or child birth weight or BMI. Only DNA methylation at cg24458009 and cg23347399 was associated with expression of PCED1B and PCDHG, respectively, in child blood, and DNA methylation at cg27193519 was associated with expression of TFAP4, ZNF500, PPL, and ANKS3 in child subcutaneous adipose tissue.CONCLUSIONS: We observed multiple associations of maternal glycemic index and load during pregnancy with cord blood DNA methylation, mostly in mothers with overweight/obesity; some of these CpGs were associated with gene expression. Additional studies are required to further explore functionality, uncover causality, and study pathways to offspring health.

AB - OBJECTIVE: Suboptimal nutrition in pregnancy is associated with worse offspring cardiometabolic health. DNA methylation may be an underlying mechanism. We meta-analyzed epigenome-wide association studies (EWAS) of maternal dietary glycemic index and load with cord blood DNA methylation.RESEARCH DESIGN AND METHODS: We calculated maternal glycemic index and load from food frequency questionnaires and ran EWAS on cord blood DNA methylation in 2,003 mother-offspring pairs from three cohorts. Analyses were additionally stratified by maternal BMI categories. We looked-up the findings in EWAS of maternal glycemic traits and BMI as well as in EWAS of birth weight and child BMI. We examined associations with gene expression in child blood in the online Human Early Life Exposome eQTM catalog and in 223 adipose tissue samples.RESULTS: Maternal glycemic index and load were associated with cord blood DNA methylation at 41 cytosine-phosphate-guanine sites (CpGs, P < 1.17 × 10-7), mostly in mothers with overweight/obesity. We did not observe overlap with CpGs associated with maternal glycemic traits, BMI, or child birth weight or BMI. Only DNA methylation at cg24458009 and cg23347399 was associated with expression of PCED1B and PCDHG, respectively, in child blood, and DNA methylation at cg27193519 was associated with expression of TFAP4, ZNF500, PPL, and ANKS3 in child subcutaneous adipose tissue.CONCLUSIONS: We observed multiple associations of maternal glycemic index and load during pregnancy with cord blood DNA methylation, mostly in mothers with overweight/obesity; some of these CpGs were associated with gene expression. Additional studies are required to further explore functionality, uncover causality, and study pathways to offspring health.

U2 - 10.2337/dc21-2662

DO - 10.2337/dc21-2662

M3 - Article (Academic Journal)

C2 - 35708509

SN - 0149-5992

VL - 45

SP - 1822

EP - 1832

JO - Diabetes Care

JF - Diabetes Care

IS - 8

ER -

Maternal Dietary Glycemic Index and Glycemic Load in Pregnancy and Offspring Cord Blood DNA Methylation

Abstract

Bibliographical note

UN SDGs

Access to Document

Handle.net

Fingerprint

Cite this