Maturation of a recurrent excitatory neocortical circuit by experience-dependent unsilencing of newly-formed dendritic spines

MC Ashby, JT Isaac

Research output: Contribution to journalArticle (Academic Journal)peer-review

41 Citations (Scopus)
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Abstract

Local recurrent excitatory circuits are ubiquitous in neocortex, yet little is known about their development or architecture. Here we introduce a quantitative technique for efficient single-cell resolution circuit mapping using 2-photon (2P) glutamate uncaging and analyze experience-dependent neonatal development of the layer 4 barrel cortex local excitatory circuit. We show that sensory experience specifically drives a 3-fold increase in connectivity at postnatal day (P) 9, producing a highly recurrent network. A profound dendritic spinogenesis occurs concurrent with the connectivity increase, but this is not experience dependent. However, in experience-deprived cortex, a much greater proportion of spines lack postsynaptic AMPA receptors (AMPARs) and synaptic connectivity via NMDA receptors (NMDARs) is the same as in normally developing cortex. Thus we describe a approach for quantitative circuit mapping and show that sensory experience sculpts an intrinsically developing template network, which is based on NMDAR-only synapses, by driving AMPARs into newly formed silent spines.
Translated title of the contributionMaturation of a recurrent excitatory neocortical circuit by experience-dependent unsilencing of newly-formed dendritic spines
Original languageEnglish
Pages (from-to)510 - 521
Number of pages12
JournalNeuron
Volume70
Issue number3
DOIs
Publication statusPublished - 12 May 2011

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