Measuring kinetics and potency of hERG block for CiPA

Monique J. Windley, Najah Abi-Gerges, Bernard Fermini, Jules C. Hancox, Jamie I. Vandenberg, Adam P. Hill

Research output: Contribution to journalArticle (Academic Journal)

22 Citations (Scopus)
249 Downloads (Pure)

Abstract

AbstractIntroduction The Comprehensive in vitro Proarrhythmic Assay (CiPA) aims to update current cardiac safety testing to better evaluate arrhythmic risk. A central theme of CiPA is the use of in silico approaches to risk prediction incorporating models of drug binding to hERG. To parameterize these models, accurate in vitro measurement of potency and kinetics of block is required. The Ion Channel Working Group was tasked with: i) selecting a protocol that could measure kinetics of block and was easily implementable on automated platforms for future rollout in industry and ii) acquiring a reference dataset using the standardized protocol. Methods Data were acquired using a ‘step depolarisation’ protocol using manual patch-clamp at ambient temperature. Results Potency, kinetics and trapping characteristics of hERG block for the CiPA training panel of twelve drugs were measured. Timecourse of block and trapping characteristics could be reliably measured if the time constant for onset of block was between ~ 500 ms and ~ 15 s. Seven drugs, however had time courses of block faster than this cut-off. Discussion Here we describe the implementation of the standardized protocol for measurement of kinetics and potency of hERG block for CiPA. The results highlight the challenges in identifying a single protocol to measure hERG block over a range of kinetics. The dataset from this study is being used by the In Silico Working Group to develop models of drug binding for risk prediction and is freely available as a ‘gold standard’ ambient temperature dataset to evaluate variability across high throughput platforms.
Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalJournal of Pharmacological and Toxicological Methods
Volume87
Early online date10 Feb 2017
DOIs
Publication statusPublished - Sep 2017

Keywords

  • Acquired long QT
  • CiPA
  • Drug screening
  • hERG
  • Proarrhythmic risk

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