Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader

Shaolin Liang; Andrew B Kinghorn; Margaritis Voliotis; Julia K Prague; Johannes D Veldhuis; Krasimira Tsaneva-Atanasova; Craig A McArdle; Raymond H W Li; Anthony E G Cass; Waljit S Dhillo; Julian A Tanner

doi:10.1038/s41467-019-08799-6

Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader

Shaolin Liang, Andrew B Kinghorn, Margaritis Voliotis, Julia K Prague, Johannes D Veldhuis, Krasimira Tsaneva-Atanasova, Craig A McArdle, Raymond H W Li, Anthony E G Cass, Waljit S Dhillo, Julian A Tanner

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Abstract

Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.

Original language	English
Article number	852
Number of pages	10
Journal	Nature Communications
Volume	10
DOIs	https://doi.org/10.1038/s41467-019-08799-6
Publication status	Published - 20 Feb 2019

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Engineering Mathematics Research Group

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title = "Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader",

abstract = "Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.",

author = "Shaolin Liang and Kinghorn, {Andrew B} and Margaritis Voliotis and Prague, {Julia K} and Veldhuis, {Johannes D} and Krasimira Tsaneva-Atanasova and McArdle, {Craig A} and Li, {Raymond H W} and Cass, {Anthony E G} and Dhillo, {Waljit S} and Tanner, {Julian A}",

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AU - Liang, Shaolin

AU - Kinghorn, Andrew B

AU - Voliotis, Margaritis

AU - Prague, Julia K

AU - Veldhuis, Johannes D

AU - Tsaneva-Atanasova, Krasimira

AU - McArdle, Craig A

AU - Li, Raymond H W

AU - Cass, Anthony E G

AU - Dhillo, Waljit S

AU - Tanner, Julian A

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AB - Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.

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JO - Nature Communications

JF - Nature Communications

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Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader

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Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader

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