Measuring screw-sense preference in a helical oligomer by comparison of 13C NMR signal separation at slow and fast exchange

Jordi Solà, Gareth A. Morris, Jonathan Clayden*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

55 Citations (Scopus)

Abstract

While an unequal population of rapidly interconverting left- and right-handed conformers of a helical oligomer can be detected by circular dichroism, precise quantification of a conformer ratio has not previously been achieved. We demonstrate, using a set of labeled peptide analogues, that simple analysis of peak separation in their 13C NMR spectra at slow and fast exchange allows an accurate value for the ratio of helical conformers to be obtained. The method reports the ratio of conformers at the site of the label and can therefore be used to investigate local variations in helical conformational control.

Original languageEnglish
Pages (from-to)3712-3715
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number11
Early online date25 Feb 2011
DOIs
Publication statusPublished - 23 Mar 2011

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