Metabolomics: a valuable tool for stem cell monitoring in regenerative medicine

Laura E. McNamara; Terje Sjöström; R. M. Dominic Meek; Richard O. C. Oreffo; Bo Su; Matthew J. Dalby; Karl E. V. Burgess

doi:10.1098/rsif.2012.0169

Metabolomics: a valuable tool for stem cell monitoring in regenerative medicine

Laura E. McNamara^*, Terje Sjöström, R. M. Dominic Meek, Richard O. C. Oreffo, Bo Su, Matthew J. Dalby, Karl E. V. Burgess

^*Corresponding author for this work

Research output: Contribution to journal › Review article (Academic Journal) › peer-review

25 Citations (Scopus)

Abstract

Metabolomics is a method for investigation of changes in the global metabolite profile of cells. This paper discusses the technical application of the approach, considering metabolite extraction, separation, mass spectrometry and data interpretation. A particular focus is on the application of metabolomics to the study of stem cell physiology in the context of biomaterials and regenerative medicine. Case studies are used to illustrate key points, focusing on the use of metabolomics in the examination of mesenchymal stem cell responses to titania-nanopillared substrata designed for orthopaedic applications.

Original language	English
Pages (from-to)	1713-1724
Number of pages	12
Journal	Journal of the Royal Society Interface
Volume	9
Issue number	73
DOIs	https://doi.org/10.1098/rsif.2012.0169
Publication status	Published - 7 Aug 2012

Keywords

biomaterials
MASS-SPECTROMETRY DATA
regenerative medicine
DIFFERENTIATION
CHROMATOGRAPHY
PROFILE
stem cells
mass spectrometry
titanium
IDENTIFICATION
EXPRESSION
TENSION
metabolomics

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MICRO- AND NANO-PATTERNING OF TITANIUM SURFACES FOR OPTIMAL OSSEOINTERGRATION OF ORTHOPAEDIC IMPLANTS
Su, B. (Principal Investigator)
1/06/09 → 1/06/12
Project: Research

Cite this

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title = "Metabolomics: a valuable tool for stem cell monitoring in regenerative medicine",

abstract = "Metabolomics is a method for investigation of changes in the global metabolite profile of cells. This paper discusses the technical application of the approach, considering metabolite extraction, separation, mass spectrometry and data interpretation. A particular focus is on the application of metabolomics to the study of stem cell physiology in the context of biomaterials and regenerative medicine. Case studies are used to illustrate key points, focusing on the use of metabolomics in the examination of mesenchymal stem cell responses to titania-nanopillared substrata designed for orthopaedic applications.",

keywords = "biomaterials, MASS-SPECTROMETRY DATA, regenerative medicine, DIFFERENTIATION, CHROMATOGRAPHY, PROFILE, stem cells, mass spectrometry, titanium, IDENTIFICATION, EXPRESSION, TENSION, metabolomics",

author = "McNamara, {Laura E.} and Terje Sj{\"o}str{\"o}m and Meek, {R. M. Dominic} and Oreffo, {Richard O. C.} and Bo Su and Dalby, {Matthew J.} and Burgess, {Karl E. V.}",

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doi = "10.1098/rsif.2012.0169",

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AU - McNamara, Laura E.

AU - Sjöström, Terje

AU - Meek, R. M. Dominic

AU - Oreffo, Richard O. C.

AU - Su, Bo

AU - Dalby, Matthew J.

AU - Burgess, Karl E. V.

PY - 2012/8/7

Y1 - 2012/8/7

N2 - Metabolomics is a method for investigation of changes in the global metabolite profile of cells. This paper discusses the technical application of the approach, considering metabolite extraction, separation, mass spectrometry and data interpretation. A particular focus is on the application of metabolomics to the study of stem cell physiology in the context of biomaterials and regenerative medicine. Case studies are used to illustrate key points, focusing on the use of metabolomics in the examination of mesenchymal stem cell responses to titania-nanopillared substrata designed for orthopaedic applications.

AB - Metabolomics is a method for investigation of changes in the global metabolite profile of cells. This paper discusses the technical application of the approach, considering metabolite extraction, separation, mass spectrometry and data interpretation. A particular focus is on the application of metabolomics to the study of stem cell physiology in the context of biomaterials and regenerative medicine. Case studies are used to illustrate key points, focusing on the use of metabolomics in the examination of mesenchymal stem cell responses to titania-nanopillared substrata designed for orthopaedic applications.

KW - biomaterials

KW - MASS-SPECTROMETRY DATA

KW - regenerative medicine

KW - DIFFERENTIATION

KW - CHROMATOGRAPHY

KW - PROFILE

KW - stem cells

KW - mass spectrometry

KW - titanium

KW - IDENTIFICATION

KW - EXPRESSION

KW - TENSION

KW - metabolomics

U2 - 10.1098/rsif.2012.0169

DO - 10.1098/rsif.2012.0169

M3 - Review article (Academic Journal)

C2 - 22628210

SN - 1742-5689

VL - 9

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EP - 1724

JO - Journal of the Royal Society Interface

JF - Journal of the Royal Society Interface

IS - 73

ER -

Metabolomics: a valuable tool for stem cell monitoring in regenerative medicine

Abstract

Keywords

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MICRO- AND NANO-PATTERNING OF TITANIUM SURFACES FOR OPTIMAL OSSEOINTERGRATION OF ORTHOPAEDIC IMPLANTS

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