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Metagenomic insights into diazotrophic communities across Arctic glacier forefields

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Metagenomic insights into diazotrophic communities across Arctic glacier forefields. / Nash, Maisie V.; Anesio, Alexandre M.; Barker, Gary; Tranter, Martyn; Varliero, Gilda; Eloe-Fadrosh, Emiley A.; Nielsen, Torben; Turpin-Jelfs, Thomas; Benning, Liane G.; Sánchez-Baracaldo, Patricia.

In: FEMS Microbiology Ecology, Vol. 94, No. 9, fiy114, 01.09.2018.

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Nash, Maisie V. ; Anesio, Alexandre M. ; Barker, Gary ; Tranter, Martyn ; Varliero, Gilda ; Eloe-Fadrosh, Emiley A. ; Nielsen, Torben ; Turpin-Jelfs, Thomas ; Benning, Liane G. ; Sánchez-Baracaldo, Patricia. / Metagenomic insights into diazotrophic communities across Arctic glacier forefields. In: FEMS Microbiology Ecology. 2018 ; Vol. 94, No. 9.

Bibtex

@article{6290fe83f83844878448bd08c6e2b237,
title = "Metagenomic insights into diazotrophic communities across Arctic glacier forefields",
abstract = "Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes.",
keywords = "Nitrogen fixation, Metagenomics, Forefield, Arctic, Diversity, Diazotrophs",
author = "Nash, {Maisie V.} and Anesio, {Alexandre M.} and Gary Barker and Martyn Tranter and Gilda Varliero and Eloe-Fadrosh, {Emiley A.} and Torben Nielsen and Thomas Turpin-Jelfs and Benning, {Liane G.} and Patricia S{\'a}nchez-Baracaldo",
year = "2018",
month = "9",
day = "1",
doi = "10.1093/femsec/fiy114",
language = "English",
volume = "94",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "9",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Metagenomic insights into diazotrophic communities across Arctic glacier forefields

AU - Nash, Maisie V.

AU - Anesio, Alexandre M.

AU - Barker, Gary

AU - Tranter, Martyn

AU - Varliero, Gilda

AU - Eloe-Fadrosh, Emiley A.

AU - Nielsen, Torben

AU - Turpin-Jelfs, Thomas

AU - Benning, Liane G.

AU - Sánchez-Baracaldo, Patricia

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes.

AB - Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes.

KW - Nitrogen fixation

KW - Metagenomics

KW - Forefield

KW - Arctic

KW - Diversity

KW - Diazotrophs

UR - http://www.scopus.com/inward/record.url?scp=85050825953&partnerID=8YFLogxK

U2 - 10.1093/femsec/fiy114

DO - 10.1093/femsec/fiy114

M3 - Article

C2 - 29901729

AN - SCOPUS:85050825953

VL - 94

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 9

M1 - fiy114

ER -