Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys2His2 zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc Z cobalt c copper c calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations.
|Translated title of the contribution||Metal Binding to a zinc-finger peptide: a comparison between solution and gas phase|
|Publication status||Published - 20 Sep 2010|