Method development for the determination of free and esterified sterols in button mushrooms (Agaricus bisporus)

Simon Hammann, Walter Vetter*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

16 Citations (Scopus)

Abstract

Ergosterol is the major sterol in button mushrooms (Agaricus bisporus) and can occur as free alcohol or esterified with fatty acids (ergosteryl esters). In this study, gas chromatography with mass spectrometry in the selected ion monitoring mode (GC/MS-SIM) was used to determine ergosterol and ergosteryl esters as well as other sterols and steryl esters in button mushrooms. Different quality control measures were established and sample preparation procedures were compared to prevent the formation of artifacts and the degradation of ergosteryl esters. The final method was then used for the determination of ergosterol (443 ± 44 mg/100 g dry matter (d.m.)) and esterified ergosterol (12 ± 6 mg/100 g d.m.) in button mushroom samples (n = 4). While the free sterol fraction was vastly dominated by ergosterol (∼90% of five sterols in total), the steryl ester fraction was more diversified (nine sterols in total, ergosterol ∼55%) and consisted primarily of linoleic acid esters.

Original languageEnglish
Pages (from-to)3437-3444
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number17
DOIs
Publication statusPublished - 4 May 2016

Keywords

  • Agaricus bisporus
  • Gas chromatography
  • Lipolysis
  • Mass spectrometry
  • Method development
  • Sterol ester

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