Methodologies for the airbrush application of MALDI matrices

Xander Warren, David J. Mitchell, Paul Gates*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

7 Citations (Scopus)
388 Downloads (Pure)


There is still a need to develop reliable and robust matrix deposition methods for matrix-assisted laser desorption/ionisation mass spectrometry that are applicable to a range of matrices, solvents and analyte types. This paper presents a robust methodology for the airbrush application of matrices along with the implications of varying the set-up and airbrush parameters. A small number of organic analytes and metal salts are analysed in both positive and negative ion modes to exemplify this methodology. In the analyses with the airbrush deposited matrices, performance was enhanced when compared to standard pipette deposition with the need for a search for sweat spots greatly diminished due to the increase homogeneity of the matrix surface and resultant analyte spots. As expected, the graphite matrices were shown to specifically outperformed the organic matrices in negative ion mode.
Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalEuropean Journal of Mass Spectrometry
Issue number1
Early online date16 Jan 2018
Publication statusPublished - 1 Feb 2018


  • airbrush
  • colloidal graphite
  • matrix
  • Matrix-assisted laser desorption
  • traditional matrices


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