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Methodologies for the airbrush application of MALDI matrices

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalEuropean Journal of Mass Spectrometry
Volume24
Issue number1
Early online date16 Jan 2018
DOIs
DateAccepted/In press - 1 Dec 2017
DateE-pub ahead of print - 16 Jan 2018
DatePublished (current) - 1 Feb 2018

Abstract

There is still a need to develop reliable and robust matrix deposition methods for matrix-assisted laser desorption/ionisation mass spectrometry that are applicable to a range of matrices, solvents and analyte types. This paper presents a robust methodology for the airbrush application of matrices along with the implications of varying the set-up and airbrush parameters. A small number of organic analytes and metal salts are analysed in both positive and negative ion modes to exemplify this methodology. In the analyses with the airbrush deposited matrices, performance was enhanced when compared to standard pipette deposition with the need for a search for sweat spots greatly diminished due to the increase homogeneity of the matrix surface and resultant analyte spots. As expected, the graphite matrices were shown to specifically outperformed the organic matrices in negative ion mode.

    Research areas

  • airbrush, colloidal graphite, matrix, Matrix-assisted laser desorption, traditional matrices

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  • Full-text PDF (accepted author manuscript)

    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Sage at http://journals.sagepub.com/doi/10.1177/1469066717750031 . Please refer to any applicable terms of use of the publisher.

    Accepted author manuscript, 491 KB, PDF document

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