Methods for imaging DNA in liquid with lateral molecular-force microscopy

RL Harniman, JA Vicary, JKH Hörber, L Picco, MJ Miles, M Antognozzi

Research output: Contribution to journalArticle (Academic Journal)

16 Citations (Scopus)

Abstract

Shear force microscopy is not normally associated with the imaging of biomolecules in a liquid environment. Here we show that the recently developed scattered evanescent wave (SEW) detection system, combined with custom-designed vertically oriented cantilevers (VOCs), can reliably produce true non-contact images in liquid of DNA molecules. The range of cantilever spring constants for successful shear force imaging was experimentally identified between 0.05 and 0.09 N/m. Images of DNA adsorbed on mica in distilled water were obtained at scan rates of 8000 pixels/s. A new constant-height force mapping mode for VOCs is also presented. This method is shown to control the vertical position of the tip in the sample plane with better than 1 nm accuracy. The force mode is demonstrated by mapping the shear force above DNA
Original languageEnglish
Article number085703
JournalNanotechnology
Volume23
Issue number8
DOIs
Publication statusPublished - Feb 2012

Bibliographical note

Publisher: IOPP

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  • Projects

    ULTRA HIGH SPEEDS NON-CONTACT FORCE MICROSCOPY

    Miles, M. J.

    1/10/041/10/09

    Project: Research

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