Microcontact printing of DNA molecules

SA Lange, V Benes, DP Kern, JKH Hoerber, A Bernard

Research output: Contribution to journalArticle (Academic Journal)peer-review

174 Citations (Scopus)

Abstract

The controlled placement of DNA molecules onto solid surfaces is the first step in the fabrication of DNA arrays. The sequential deposition of tiny drops containing the probe DNA fragments using arrays of spotting needles or ink jet nozzles has become a standard. However, a caveat of liquid spotting is the drying of the deposited drop because this creates the typical inhomogeneities, i.e., rims around the spot. Another drawback is that each DNA array is an original and has to be fabricated individually. Microcontact printing is a versatile technique to place proteins onto different target surfaces in uniformly patterned monolayers with high lateral resolution. Here, we show for the first time that DNA can also be printed with equally high resolution in the submicrometer range using an elastomeric stamp with chemically tailored surface. Two regimes for the transfer of the molecules were observed. Finally, microcontact printing of an array of DNA probes onto a solid support and its use in a subsequent hybridization assay was demonstrated.
Translated title of the contributionMicrocontact printing of DNA molecules
Original languageEnglish
Pages (from-to)1641 - 1647
Number of pages7
JournalAnalytical Chemistry
Volume76(6)
Publication statusPublished - 2004

Bibliographical note

Publisher: Amer. Chemical Society

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