Microfluidic formation of proteinosomes

Martina Ugrinic, Adrian Zambrano, Simon Berger, Stephen Mann, T. Y.Dora Tang*, Andrew Demello

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

15 Citations (Scopus)
253 Downloads (Pure)


Herein we describe a novel microfluidic method for the generation of proteinosome micro-droplets, based on bovine serum albumin and glucose oxidase conjugated to PNIPAAm chains. The size of such water-in-oil droplets is regulated via control of the input reagent flow rate, with generated proteinosome populations exhibiting narrower size distributions than those observed when using standard bulk methodologies. Importantly, proteinosomes transferred from an oil to an aqueous-environment remain intact, become fully hydrated and exhibit an increase in average size. Moreover, functional proteinosomes prepared via microfluidics exhibit lower Km values and higher enzymatic activities than proteinosomes produced by bulk methodologies.

Original languageEnglish
Pages (from-to)287-290
Number of pages4
JournalChemical Communications
Issue number3
Early online date6 Dec 2017
Publication statusPublished - 1 Jan 2018

Structured keywords

  • BrisSynBio
  • Bristol BioDesign Institute

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