Milligram quantity preparation of RNA from a marine invertebrate with a high fluid content

Harry J Witchel, Norman J Maitland, Robert W Meech

Research output: Contribution to journalArticle (Academic Journal)

1 Citation (Scopus)

Abstract

Preparation of milligram quantities of RNA from mesoglea containing marine invertebrates presents unique difficulties when using the traditional methods that begin with detergent-based orguanidinium-based extraction buffers.
Original languageEnglish
Pages (from-to)1024-1026
Number of pages3
JournalBiotechniques
Volume21
Issue number6
Publication statusPublished - 1 Dec 1996

Keywords

  • Animals
  • Blotting, Northern
  • Buffers
  • Chemical Precipitation
  • Cnidaria
  • Electrophoresis, Agar Gel
  • Lithium Chloride
  • RNA

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