Abstract
We have analysed the behaviour of minimal residual disease (MRD) after allogeneic bone marrow transplantation (allo-BMT) in 71 children with acute lymphoblastic leukaemia (ALL). The method relied on PCR of IgH, TCRdelta and/or TCRgamma gene rearrangements followed by electrophoretic size resolution and allele-specific oligoprobing. Patients were similarly conditioned; 55 received marrow from unrelated donors and 16 from related donors. MRD was assessed at various time-points up to 24 months after BMT. Three children were not evaluable due to transplant-related mortality. MRD was detected in 28/32 patients (88%) who relapsed post-BMT; 16 were positive at all times and 12 were initially negative but became positive at a median of 3 months (range 1.5-11) prior to relapse. In contrast, only eight of 36 (22%) patients who remained in continuing complete remission (CCR) (median follow-up 43 months, range 20-94) showed MRD at any time after BMT (P
Original language | English |
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Pages (from-to) | 860-71 |
Number of pages | 12 |
Journal | British Journal of Haematology |
Volume | 102 |
Issue number | 3 |
DOIs | |
Publication status | Published - Aug 1998 |
Keywords
- Adolescent
- Bone Marrow Transplantation
- Child
- Child, Preschool
- Humans
- Infant
- Infant, Newborn
- Neoplasm, Residual
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
- Recurrence
- Transplantation, Homologous