miR-186 inhibits muscle cell differentiation through myogenin regulation

Antonis Antoniou, Nikolaos P Mastroyiannopoulos, James B Uney, Leonidas A Phylactou

Research output: Contribution to journalArticle (Academic Journal)

37 Citations (Scopus)

Abstract

The complex process of skeletal muscle differentiation is organized by the myogenic regulatory factors (MRFs), Myf5, MyoD, Myf6, and myogenin, where myogenin plays a critical role in the regulation of the final stage of muscle differentiation. In an effort to investigate the role microRNAs (miRNAs) play in regulating myogenin, a bioinformatics approach was used and six miRNAs (miR-182, miR-186, miR-135, miR-491, miR-329, and miR-96) were predicted to bind the myogenin 3'-untranslated region (UTR). However, luciferase assays showed only miR-186 inhibited translation and 3'-UTR mutagenesis analysis confirmed this interaction was specific. Interestingly, the expression of miR-186 mirrored that of its host gene, ZRANB2, during development. Functional studies demonstrated that miR-186 overexpression inhibited the differentiation of C2C12 and primary muscle cells. Our findings therefore identify miR-186 as a novel regulator of myogenic differentiation.

Original languageEnglish
Pages (from-to)3923-35
Number of pages13
JournalJournal of Biological Chemistry
Volume289
Issue number7
DOIs
Publication statusPublished - 14 Feb 2014

Keywords

  • 3' Untranslated Regions
  • Animals
  • Cell Differentiation
  • Cell Line
  • Mice
  • MicroRNAs
  • Muscle, Skeletal
  • Myogenin
  • RNA-Binding Proteins

Fingerprint Dive into the research topics of 'miR-186 inhibits muscle cell differentiation through myogenin regulation'. Together they form a unique fingerprint.

Cite this