Mitochondrial calcium as a key regulator of mitochondrial ATP production in mammalian cells

EJ Griffiths, GA Rutter

Research output: Contribution to journalArticle (Academic Journal)peer-review

190 Citations (Scopus)

Abstract

Mitochondrial Ca(2+) transport was initially considered importantly only in buffering of cytosolic Ca(2+) by acting as a "sink" under conditions of Ca(2+) overload. The main regulator of ATP production was considered to be the relative concentrations of high energy phosphates. However, work by Denton and McCormack in the 1970's and 1980's showed that free intramitochondrial Ca(2+) ([Ca(2+)](m)) activated dehydrogenase enzymes in mitochondria, leading to increased NADH and hence ATP production. This lead them to propose a scheme, subsequently termed a "parallel activation model" whereby increases in energy demand, such as hormonal stimulation or increased workload in muscle, produced an increase in cytosolic [Ca(2+)] that was relayed by the mitochondrial Ca(2+) transporters into the matrix to give an increase in [Ca(2+)](m). This then stimulated energy production to meet the increased energy demand. With the development of methods for measuring [Ca(2+)](m) in living cells that proved [Ca(2+)](m) changed over a dynamic physiological range rather than simply soaking up excess cytosolic [Ca(2+)], this model has now gained widespread acceptance. However, work by ourselves and others using targeted probes to measure changes in both [Ca(2+)] and [ATP] in different cell compartments has revealed variations in the interrelationships between these two in different tissues, suggesting that metabolic regulation by Ca(2+) is finely tuned to the demands and function of the individual organ.
Translated title of the contributionMitochondrial calcium as a key regulator of mitochondrial ATP production in mammalian cells
Original languageEnglish
Pages (from-to)1324 - 1333
Number of pages10
JournalBiochimica et Biophysica Acta (BBA) - Bioenergetics
Volume1787 (11)
DOIs
Publication statusPublished - Nov 2009

Bibliographical note

Publisher: Elsevier
Other: First published online 3rd February 2009

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