Mobilisation of jerboa kidney gene networks during dehydration and opportunistic rehydration

Benjamin T Gillard; Nabil Amor; Fernando Alvira Iraizoz; Audrys G Pauža; Colin Campbell; Michael P Greenwood; Abdulaziz N Alagaili; David Murphy

doi:10.1016/j.isci.2023.107574

Mobilisation of jerboa kidney gene networks during dehydration and opportunistic rehydration

Benjamin T Gillard, Nabil Amor, Fernando Alvira Iraizoz, Audrys G Pauža, Colin Campbell, Michael P Greenwood, Abdulaziz N Alagaili, David Murphy^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

Desert animals have evolved systems that enable them to thrive under dry conditions. Focusing on the kidney, we have investigated the transcriptomic adaptations that enable a desert rodent, the Lesser Egyptian Jerboa (Jaculus jaculus), to withstand water deprivation and opportunistic rehydration. Analysis of the whole kidney transcriptome showed many differentially expressed genes in the Jerboa kidney, 6.4% of genes following dehydration and an even greater number (36.2%) following rehydration compared to control. Genes correlated with the rehydration condition included many ribosomal protein coding genes suggesting a concerted effort to accelerate protein synthesis when water is made available. We identify an increase in TGF-beta signaling antagonists in dehydration (e.g., GREM2). We also describe expression of multiple aquaporin and solute carrier transporters mapped to specific nephron segments. The desert adapted renal transcriptome presented here is a valuable resource to expand our understanding of osmoregulation beyond that derived from model organisms.

Original language	English
Article number	107574
Journal	iScience
Volume	26
Issue number	9
DOIs	https://doi.org/10.1016/j.isci.2023.107574
Publication status	Published - 9 Aug 2023

Bibliographical note

Funding Information:
This research was generously supported by grants from the Leverhulme Trust ( RPG-2017-287 ) to B.T.G., F.A.-I., D.M. and M.P.G., the BBSRC ( BB/R016879/1 ) to D.M., C.C. and M.P.G., the EPSRC ( EP/K008250/1 ) to C.C. and D.M., and also funded by Researchers Supporting Project number ( RSPD2023R602 ), King Saud University , Riyadh, Saudi Arabia. Students were supported by grants from the Biotechnology and Biological Sciences Research Council -SWBio DTP program ( BBSRC BB/M009122/1 ) to B.T.G. and the British Heart Foundation ( BHF FS/17/60/33474 ) to A.G.P.

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© 2023 The Author(s)

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title = "Mobilisation of jerboa kidney gene networks during dehydration and opportunistic rehydration",

abstract = "Desert animals have evolved systems that enable them to thrive under dry conditions. Focusing on the kidney, we have investigated the transcriptomic adaptations that enable a desert rodent, the Lesser Egyptian Jerboa (Jaculus jaculus), to withstand water deprivation and opportunistic rehydration. Analysis of the whole kidney transcriptome showed many differentially expressed genes in the Jerboa kidney, 6.4% of genes following dehydration and an even greater number (36.2%) following rehydration compared to control. Genes correlated with the rehydration condition included many ribosomal protein coding genes suggesting a concerted effort to accelerate protein synthesis when water is made available. We identify an increase in TGF-beta signaling antagonists in dehydration (e.g., GREM2). We also describe expression of multiple aquaporin and solute carrier transporters mapped to specific nephron segments. The desert adapted renal transcriptome presented here is a valuable resource to expand our understanding of osmoregulation beyond that derived from model organisms.",

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note = "Funding Information: This research was generously supported by grants from the Leverhulme Trust ( RPG-2017-287 ) to B.T.G., F.A.-I., D.M. and M.P.G., the BBSRC ( BB/R016879/1 ) to D.M., C.C. and M.P.G., the EPSRC ( EP/K008250/1 ) to C.C. and D.M., and also funded by Researchers Supporting Project number ( RSPD2023R602 ), King Saud University , Riyadh, Saudi Arabia. Students were supported by grants from the Biotechnology and Biological Sciences Research Council -SWBio DTP program ( BBSRC BB/M009122/1 ) to B.T.G. and the British Heart Foundation ( BHF FS/17/60/33474 ) to A.G.P. Publisher Copyright: {\textcopyright} 2023 The Author(s)",

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AU - Greenwood, Michael P

AU - Alagaili, Abdulaziz N

AU - Murphy, David

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N2 - Desert animals have evolved systems that enable them to thrive under dry conditions. Focusing on the kidney, we have investigated the transcriptomic adaptations that enable a desert rodent, the Lesser Egyptian Jerboa (Jaculus jaculus), to withstand water deprivation and opportunistic rehydration. Analysis of the whole kidney transcriptome showed many differentially expressed genes in the Jerboa kidney, 6.4% of genes following dehydration and an even greater number (36.2%) following rehydration compared to control. Genes correlated with the rehydration condition included many ribosomal protein coding genes suggesting a concerted effort to accelerate protein synthesis when water is made available. We identify an increase in TGF-beta signaling antagonists in dehydration (e.g., GREM2). We also describe expression of multiple aquaporin and solute carrier transporters mapped to specific nephron segments. The desert adapted renal transcriptome presented here is a valuable resource to expand our understanding of osmoregulation beyond that derived from model organisms.

AB - Desert animals have evolved systems that enable them to thrive under dry conditions. Focusing on the kidney, we have investigated the transcriptomic adaptations that enable a desert rodent, the Lesser Egyptian Jerboa (Jaculus jaculus), to withstand water deprivation and opportunistic rehydration. Analysis of the whole kidney transcriptome showed many differentially expressed genes in the Jerboa kidney, 6.4% of genes following dehydration and an even greater number (36.2%) following rehydration compared to control. Genes correlated with the rehydration condition included many ribosomal protein coding genes suggesting a concerted effort to accelerate protein synthesis when water is made available. We identify an increase in TGF-beta signaling antagonists in dehydration (e.g., GREM2). We also describe expression of multiple aquaporin and solute carrier transporters mapped to specific nephron segments. The desert adapted renal transcriptome presented here is a valuable resource to expand our understanding of osmoregulation beyond that derived from model organisms.

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DO - 10.1016/j.isci.2023.107574

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Mobilisation of jerboa kidney gene networks during dehydration and opportunistic rehydration

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An Active Learning Approach to Network Inference

The Transcriptomics of the Hypothalamic-Kidney Axis in a Desert Rodent

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Mobilisation of jerboa kidney gene networks during dehydration and opportunistic rehydration

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The Transcriptomics of the Hypothalamic-Kidney Axis in a Desert Rodent

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