Modeling Podocyte Biology Using Drosophila Nephrocytes

Paul S Hartley*, Richard J Coward

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

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Abstract

Vertebrate podocytes are kidney glomerular cells critically required for normal renal filtration. To fulfill their role, podocytes form molecular sieves known as slit diaphragms that contribute to the glomerular filtration barrier. The disruption of podocyte biology or slit diaphragm formation in humans is a precursor to albuminuria, renal failure, and cardiovascular morbidity. Due to genetic and functional similarities, the nephrocytes of Drosophila are increasingly used to model the genetic and metabolic basis of human podocyte biology. They have the advantage that they are a much quicker system to study compared to other murine transgenic models. In this chapter we present methods to modulate and study Drosophila nephrocyte function and diaphragm formation.

Original languageEnglish
Title of host publicationDiabetic Nephropathy
Subtitle of host publicationMethods and Protocols
PublisherSpringer
Pages11-24
Number of pages14
ISBN (Electronic)978-1-4939-9841-8
ISBN (Print)978-1-4939-9840-1
DOIs
Publication statusPublished - 8 Nov 2019

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume2067
ISSN (Print)1064-3745

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