Modulation of 'A'-type K+ current by rodent and human forms of amyloid beta protein

Talitha L Kerrigan, Lucy Atkinson, Chris Peers, Hugh A Pearson

Research output: Contribution to journalArticle (Academic Journal)peer-review

19 Citations (Scopus)

Abstract

The Alzheimer's disease related peptide amyloid beta (Abeta) might have a physiological role in upregulating K channel currents in neurones. Earlier studies used the human form of Abeta1-40 on rat neurones. We sought to confirm our hypothesis by use of rat Abeta, which has no Alzheimer's association. In rat cerebellar granule neurones and HEK293 cells expressing Kv4.2 subunits, whole-cell patch clamp of K currents revealed that preincubation of cells with recombinant human or rat Abeta1-40 (10 nM for 24 h) significantly increased K channel current density. This was accompanied by increased mRNA levels for Kv4.2. These data indicate that rodent and human Abeta are effective in modulating K currents. The effectiveness of nonaggregating rat Abeta also strongly supports a physiological role for the peptide.

Original languageEnglish
Pages (from-to)839-43
Number of pages5
JournalNeuroReport
Volume19
Issue number8
DOIs
Publication statusPublished - 28 May 2008

Keywords

  • Amyloid beta-Peptides
  • Animals
  • Cell Line
  • Cerebellum
  • Humans
  • Ion Channel Gating
  • Kidney
  • Neurons
  • Patch-Clamp Techniques
  • Peptide Fragments
  • Potassium
  • RNA, Messenger
  • Rats
  • Recombinant Proteins
  • Shal Potassium Channels

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