Modulation of Higher-order Behaviour in Model Protocell Communities by Artificial Phagocytosis

Laura Rodríguez-Arco; B V V S Pavan Kumar; Mei Li; Avinash J Patil; Stephen Mann

doi:10.1002/anie.201901469

Modulation of Higher-order Behaviour in Model Protocell Communities by Artificial Phagocytosis

Laura Rodríguez-Arco, B V V S Pavan Kumar, Mei Li, Avinash J Patil, Stephen Mann^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

38 Citations (Scopus)

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Abstract

Collective behaviour in mixed populations of synthetic protocells is an unexplored area of bottom-up synthetic biology. The dynamics of a model protocell community is exploited to modulate the function and higher-order behaviour of mixed populations of bioinorganic protocells in response to a process of artificial phagocytosis. Enzyme-loaded silica colloidosomes are spontaneously engulfed by magnetic Pickering emulsion (MPE) droplets containing complementary enzyme substrates to initiate a range of processes within the host/guest protocells. Specifically, catalase, lipase, or alkaline phosphatase-filled colloidosomes are used to trigger phagocytosis-induced buoyancy, membrane reconstruction, or hydrogelation, respectively, within the MPE droplets. The results highlight the potential for exploiting surface-contact interactions between different membrane-bounded droplets to transfer and co-locate discrete chemical packages (artificial organelles) in communities of synthetic protocells.

Original language	English
Pages (from-to)	6333-6337
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	58
Issue number	19
Early online date	12 Mar 2019
DOIs	https://doi.org/10.1002/anie.201901469
Publication status	Published - 6 May 2019

Bibliographical note

Research Groups and Themes

BrisSynBio
Bristol BioDesign Institute
Inorganic & Materials

Keywords

protocells
synthetic biology
microcapsules
colloids
SYNTHETIC BIOLOGY
emulsions
enzymes

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@article{884172469a404d3189e9353bb6c417d5,

title = "Modulation of Higher-order Behaviour in Model Protocell Communities by Artificial Phagocytosis",

abstract = "Collective behaviour in mixed populations of synthetic protocells is an unexplored area of bottom-up synthetic biology. The dynamics of a model protocell community is exploited to modulate the function and higher-order behaviour of mixed populations of bioinorganic protocells in response to a process of artificial phagocytosis. Enzyme-loaded silica colloidosomes are spontaneously engulfed by magnetic Pickering emulsion (MPE) droplets containing complementary enzyme substrates to initiate a range of processes within the host/guest protocells. Specifically, catalase, lipase, or alkaline phosphatase-filled colloidosomes are used to trigger phagocytosis-induced buoyancy, membrane reconstruction, or hydrogelation, respectively, within the MPE droplets. The results highlight the potential for exploiting surface-contact interactions between different membrane-bounded droplets to transfer and co-locate discrete chemical packages (artificial organelles) in communities of synthetic protocells.",

keywords = "protocells, synthetic biology, microcapsules, colloids, SYNTHETIC BIOLOGY, emulsions, enzymes",

author = "Laura Rodr{\'i}guez-Arco and Kumar, \{B V V S Pavan\} and Mei Li and Patil, \{Avinash J\} and Stephen Mann",

note = "{\textcopyright} 2019 The Authors. Published by Wiley-VCH Verlag GmbH \& Co. KGaA.",

year = "2019",

month = may,

day = "6",

doi = "10.1002/anie.201901469",

language = "English",

volume = "58",

pages = "6333--6337",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

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TY - JOUR

T1 - Modulation of Higher-order Behaviour in Model Protocell Communities by Artificial Phagocytosis

AU - Rodríguez-Arco, Laura

AU - Kumar, B V V S Pavan

AU - Li, Mei

AU - Patil, Avinash J

AU - Mann, Stephen

PY - 2019/5/6

Y1 - 2019/5/6

N2 - Collective behaviour in mixed populations of synthetic protocells is an unexplored area of bottom-up synthetic biology. The dynamics of a model protocell community is exploited to modulate the function and higher-order behaviour of mixed populations of bioinorganic protocells in response to a process of artificial phagocytosis. Enzyme-loaded silica colloidosomes are spontaneously engulfed by magnetic Pickering emulsion (MPE) droplets containing complementary enzyme substrates to initiate a range of processes within the host/guest protocells. Specifically, catalase, lipase, or alkaline phosphatase-filled colloidosomes are used to trigger phagocytosis-induced buoyancy, membrane reconstruction, or hydrogelation, respectively, within the MPE droplets. The results highlight the potential for exploiting surface-contact interactions between different membrane-bounded droplets to transfer and co-locate discrete chemical packages (artificial organelles) in communities of synthetic protocells.

AB - Collective behaviour in mixed populations of synthetic protocells is an unexplored area of bottom-up synthetic biology. The dynamics of a model protocell community is exploited to modulate the function and higher-order behaviour of mixed populations of bioinorganic protocells in response to a process of artificial phagocytosis. Enzyme-loaded silica colloidosomes are spontaneously engulfed by magnetic Pickering emulsion (MPE) droplets containing complementary enzyme substrates to initiate a range of processes within the host/guest protocells. Specifically, catalase, lipase, or alkaline phosphatase-filled colloidosomes are used to trigger phagocytosis-induced buoyancy, membrane reconstruction, or hydrogelation, respectively, within the MPE droplets. The results highlight the potential for exploiting surface-contact interactions between different membrane-bounded droplets to transfer and co-locate discrete chemical packages (artificial organelles) in communities of synthetic protocells.

KW - protocells

KW - synthetic biology

KW - microcapsules

KW - colloids

KW - SYNTHETIC BIOLOGY

KW - emulsions

KW - enzymes

UR - https://www.scopus.com/pages/publications/85063910324

U2 - 10.1002/anie.201901469

DO - 10.1002/anie.201901469

M3 - Article (Academic Journal)

C2 - 30861271

SN - 1433-7851

VL - 58

SP - 6333

EP - 6337

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 19

ER -

Modulation of Higher-order Behaviour in Model Protocell Communities by Artificial Phagocytosis

Abstract

Bibliographical note

Research Groups and Themes

Keywords

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BrisSynBio: Bristol Centre for Synthetic Biology

Dr Avinash J Patil

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Modulation of Higher-order Behaviour in Model Protocell Communities by Artificial Phagocytosis

Abstract

Bibliographical note

Research Groups and Themes

Keywords

Access to Document

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Other files and links

Fingerprint

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BrisSynBio: Bristol Centre for Synthetic Biology

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Dr Avinash J Patil

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