Molecular cloning and phylogenetic analysis of a cDNA encoding the cat (Felis domesticus) Ig epsilon constant region

ER Weber*, CR Helps, AP Foster, ACF Perry, TJ Gruffydd-Jones, L Hall, DA Harbour, WPH Duffus, Aiden P Foster

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

11 Citations (Scopus)

Abstract

A feline splenic cDNA library was screened with a P-32-labelled cDNA probe encoding the canine IgE epsilon heavy chain subunit. A cDNA sequence of 1614 nucleotides encoding the complete feline IgE heavy chain, as well as a portion of a variable region, was identified. A search of the GenBank database revealed an identity of 82% at the nucleotide level and 76% at the amino acid level between the feline epsilon heavy chain sequence and the canine homologue. In a separate study, feline genomic DNA, isolated from whole feline embryo cells, was subjected to PCR amplification using primers based on known partial genomic DNA sequences for the feline CE gene. Following removal of an intron from the 683 bp PCR product, the coding sequence yielded an ORF of 506 bp. The DNA sequence of this PCR clone differed by a single nucleotide from the cDNA clone. This difference is silent, and therefore the proteins encoded by the two sequences are identical over the regions cloned and sequenced. Phylogenetic analysis of the constant regions of nine immunoglobulin epsilon genes revealed that the feline cDNA is most similar to the canine homologue. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)299-308
Number of pages10
JournalVeterinary Immunology and Immunopathology
Volume76
Issue number3-4
Publication statusPublished - 31 Oct 2000

Keywords

  • immunoglobulin
  • epsilon
  • feline
  • IgE
  • heavy chain
  • cDNA
  • PCR
  • RAT IMMUNOGLOBULIN-E
  • HEAVY-CHAIN
  • NUCLEOTIDE-SEQUENCE
  • C-EPSILON
  • GENES
  • EVOLUTION
  • DNA
  • RESPONSES
  • INFECTION
  • ANTIBODY

Fingerprint

Dive into the research topics of 'Molecular cloning and phylogenetic analysis of a cDNA encoding the cat (Felis domesticus) Ig epsilon constant region'. Together they form a unique fingerprint.

Cite this