Molecular Recognition of beta-O-GlcNAc Glycopeptides by a Lectin-Like Receptor: Binding Modulation by the Underlying Ser or Thr Amino Acids

Francisco Corzana, Alberto Fernandez-Tejada, Jesus H. Busto, Gururaj Joshi, Anthony P. Davis, Jesus Jimenez-Barbero, Alberto Avenoza, Jesus M. Peregrina

Research output: Contribution to journalArticle (Academic Journal)

11 Citations (Scopus)

Abstract

The binding properties of different carbohydrates and glycopeptides containing the beta-O-2-deoxy-2-(N-acetyl)-D-glucosaminyl (beta-O-GlcNAc) to a synthetically prepared lectin-like receptor have been analyzed. The study combines the use of NMR spectroscopy experiments with extensive MD simulations in explicit water. Notably, the presence of a key hydrogen bond between the receptor and the OMe group of the beta-OGlcNAc-OMe derivative appears to be responsible for the high selectivity observed for this compound. In addition, to study the effect on the binding of the underlying amino acid, we have prepared different model glycopeptides, which include the non-natural alpha-methylserine and alpha-methylthreonine as underlying amino acids. Interestingly, the presence of a methyl group decreases the affinity constant, especially in those cases in which a beta-methyl group is present. As a result, the serinecontaining glycopeptide exhibited the highest affinity constant of the glycopeptides, and the threonine derivative showed the lowest one. This low selectivity could have its origin in the difficulty to form both specific hydrogen bonds and hydrophobic (CH-pi) contacts.

Original languageEnglish
Pages (from-to)110-117
Number of pages8
JournalChemBioChem
Volume12
Issue number1
DOIs
Publication statusPublished - 3 Jan 2011

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Corzana, F., Fernandez-Tejada, A., Busto, J. H., Joshi, G., Davis, A. P., Jimenez-Barbero, J., Avenoza, A., & Peregrina, J. M. (2011). Molecular Recognition of beta-O-GlcNAc Glycopeptides by a Lectin-Like Receptor: Binding Modulation by the Underlying Ser or Thr Amino Acids. ChemBioChem, 12(1), 110-117. https://doi.org/10.1002/cbic.201000526