Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis

Ahmed M. Moustafa, Mark D. Bennett*, John Edwards, Kamran Azim, Muhammed A. Mesaik, M. Iqbal Choudhary, Pornpen Pathanasophon, Apasara Worarach, Qurban Ali, Muhammad Abubakar, Rehana Anjum

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

16 Citations (Scopus)

Abstract

A comparative genetic study of 23 field isolates and vaccine strains of Pasteurella multocida associated with haemorrhagic septicaemia cases from Pakistan and Thailand was done using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The MLST sequence type (ST) for all 20 of the 23 isolates tested was 122. The PFGE results showed one band difference between the Pakistani and the Thai isolates. Sequence type 122 is the dominant associated profile with haemorrhagic septicaemia (HS) cases in South Asia. The study supports the concept of using PFGE for short-term epidemiology and MLST for long-term epidemiology.

Original languageEnglish
Pages (from-to)986-990
Number of pages5
JournalResearch in Veterinary Science
Volume95
Issue number3
DOIs
Publication statusPublished - Dec 2013

Keywords

  • Haemorrhagic septicaemia
  • Multilocus sequence typing
  • Pakistan
  • Pasteurella multocida
  • Pulsed field gel electrophoresis
  • Thailand

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