MultiBac: From protein complex structures to synthetic viral nanosystems

Martin Pelosse, Hannah Crocker, Barbara Gorda, Paul Lemaire, Jens Rauch, Imre Berger*

*Corresponding author for this work

Research output: Contribution to journalReview article (Academic Journal)peer-review

14 Citations (Scopus)
346 Downloads (Pure)


The MultiBac baculovirus/insect cell expression vector system was conceived as a user-friendly, modular tool-kit for producing multiprotein complexes for structural biology applications. MultiBac has allowed the structure and function of many molecular machines to be elucidated, including previously inaccessible high-value drug targets. More recently, MultiBac developments have shifted to customized baculoviral genomes that are tailored for a range of applications, including synthesizing artificial proteins by genetic code expansion. We review some of these developments, including the ongoing rewiring of the MultiBac system for mammalian applications, notably CRISPR/Cas9-mediated gene editing.

Original languageEnglish
Article number99
Number of pages10
JournalBMC Biology
Publication statusPublished - 30 Oct 2017

Structured keywords

  • Bristol BioDesign Institute
  • BrisSynBio




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