MultiBac: From protein complex structures to synthetic viral nanosystems

Martin Pelosse; Hannah Crocker; Barbara Gorda; Paul Lemaire; Jens Rauch; Imre Berger

doi:10.1186/s12915-017-0447-6

MultiBac: From protein complex structures to synthetic viral nanosystems

Martin Pelosse
, Hannah Crocker
, Barbara Gorda
, Paul Lemaire
, Jens Rauch
, Imre Berger^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article (Academic Journal) › peer-review

30 Citations (Scopus)

541 Downloads (Pure)

Abstract

The MultiBac baculovirus/insect cell expression vector system was conceived as a user-friendly, modular tool-kit for producing multiprotein complexes for structural biology applications. MultiBac has allowed the structure and function of many molecular machines to be elucidated, including previously inaccessible high-value drug targets. More recently, MultiBac developments have shifted to customized baculoviral genomes that are tailored for a range of applications, including synthesizing artificial proteins by genetic code expansion. We review some of these developments, including the ongoing rewiring of the MultiBac system for mammalian applications, notably CRISPR/Cas9-mediated gene editing.

Original language	English
Article number	99
Number of pages	10
Journal	BMC Biology
Volume	15
DOIs	https://doi.org/10.1186/s12915-017-0447-6
Publication status	Published - 30 Oct 2017

Research Groups and Themes

Bristol BioDesign Institute
BrisSynBio

Keywords

SYNTHETIC BIOLOGY
synthetic biology

Access to Document

10.1186/s12915-017-0447-6

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This is the final published version of the article (version of record). It first appeared online via BMC at https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-017-0447-6#Declarations. Please refer to any applicable terms of use of the publisher.
Final published version, 3.35 MBLicence: CC BY

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AU - Pelosse, Martin

AU - Crocker, Hannah

AU - Gorda, Barbara

AU - Lemaire, Paul

AU - Rauch, Jens

AU - Berger, Imre

PY - 2017/10/30

Y1 - 2017/10/30

N2 - The MultiBac baculovirus/insect cell expression vector system was conceived as a user-friendly, modular tool-kit for producing multiprotein complexes for structural biology applications. MultiBac has allowed the structure and function of many molecular machines to be elucidated, including previously inaccessible high-value drug targets. More recently, MultiBac developments have shifted to customized baculoviral genomes that are tailored for a range of applications, including synthesizing artificial proteins by genetic code expansion. We review some of these developments, including the ongoing rewiring of the MultiBac system for mammalian applications, notably CRISPR/Cas9-mediated gene editing.

AB - The MultiBac baculovirus/insect cell expression vector system was conceived as a user-friendly, modular tool-kit for producing multiprotein complexes for structural biology applications. MultiBac has allowed the structure and function of many molecular machines to be elucidated, including previously inaccessible high-value drug targets. More recently, MultiBac developments have shifted to customized baculoviral genomes that are tailored for a range of applications, including synthesizing artificial proteins by genetic code expansion. We review some of these developments, including the ongoing rewiring of the MultiBac system for mammalian applications, notably CRISPR/Cas9-mediated gene editing.

KW - SYNTHETIC BIOLOGY

KW - synthetic biology

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DO - 10.1186/s12915-017-0447-6

M3 - Review article (Academic Journal)

C2 - 29084535

AN - SCOPUS:85032568032

SN - 1741-7007

VL - 15

JO - BMC Biology

JF - BMC Biology

M1 - 99

ER -

MultiBac: From protein complex structures to synthetic viral nanosystems

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