MultiBacMam Bimolecular Fluorescence Complementation (BiFC) tool-kit identifies new small-molecule inhibitors of the CDK5-p25 protein-protein interaction (PPI)

Itxaso Bellón-Echeverría, Jean Philippe Carralot, Andrea Araujo Del Rosario, Stephanie Kueng, Harald Mauser, Georg Schmid, Ralf Thoma*, Imre Berger

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

2 Citations (Scopus)
267 Downloads (Pure)

Abstract

Protein-protein interactions (PPIs) are at the core of virtually all biological processes in cells. Consequently, targeting PPIs is emerging at the forefront of drug discovery. Cellular assays which closely recapitulate native conditions in vivo are instrumental to understand how small molecule drugs can modulate such interactions. We have integrated MultiBacMam, a baculovirus-based mammalian gene delivery tool we developed, with bimolecular fluorescence complementation (BiFC), giving rise to a highly efficient system for assay development, identification and characterization of PPI modulators. We used our system to analyze compounds impacting on CDK5-p25 PPI, which is implicated in numerous diseases including Alzheimer's. We evaluated our tool-kit with the known inhibitor p5T, and we established a mini-screen to identify compounds that modulate this PPI in dose-response experiments. Finally, we discovered several compounds disrupting CDK5-p25 PPI, which had not been identified by other screening or structure-based methods before.

Original languageEnglish
Article number5083
Number of pages10
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 23 Mar 2018

Structured keywords

  • BrisSynBio
  • Bristol BioDesign Institute

Keywords

  • High-throughput screening
  • Kinases
  • Synthetic Biology

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