Multifunctional, self-assembling anionic peptide-lipid nanocomplexes for targeted siRNA delivery

Aristides D Tagalakis, Do Hyang D Lee, Alison S Bienemann, Haiyan Zhou, Mustafa M Munye, Luisa Saraiva, David McCarthy, Zixiu Du, Conrad A Vink, Ruhina Maeshima, Edward A White, Kenth Gustafsson, Stephen L Hart

Research output: Contribution to journalArticle (Academic Journal)peer-review

52 Citations (Scopus)

Abstract

Formulations of cationic liposomes and polymers readily self-assemble by electrostatic interactions with siRNA to form cationic nanoparticles which achieve efficient transfection and silencing in vitro. However, the utility of cationic formulations in vivo is limited due to rapid clearance from the circulation, due to their association with serum proteins, as well as systemic and cellular toxicity. These problems may be overcome with anionic formulations but they provide challenges of self-assembly and transfection efficiency. We have developed anionic, siRNA nanocomplexes utilizing anionic PEGylated liposomes and cationic targeting peptides that overcome these problems. Biophysical measurements indicated that at optimal ratios of components, anionic PEGylated nanocomplexes formed spherical particles and that, unlike cationic nanocomplexes, were resistant to aggregation in the presence of serum, and achieved significant gene silencing although their non-PEGylated anionic counterparts were less efficient. We have evaluated the utility of anionic nanoparticles for the treatment of neuronal diseases by administration to rat brains of siRNA to BACE1, a key enzyme involved in the formation of amyloid plaques. Silencing of BACE1 was achieved in vivo following a single injection of anionic nanoparticles by convection enhanced delivery and specificity of RNA interference verified by 5' RACE-PCR and Western blot analysis of protein.

Original languageEnglish
Pages (from-to)8406-15
Number of pages10
JournalBiomaterials
Volume35
Issue number29
DOIs
Publication statusPublished - 1 Sep 2014

Keywords

  • Amyloid Precursor Protein Secretases
  • Animals
  • Anions
  • Aspartic Acid Endopeptidases
  • Brain
  • Brain Diseases
  • Cell Line
  • Genetic Therapy
  • Humans
  • Lipids
  • Liposomes
  • Male
  • Nanoparticles
  • Polyethylene Glycols
  • RNA Interference
  • RNA, Small Interfering
  • Rats
  • Rats, Wistar
  • Transfection

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