Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

Liang Chen, Kalyan D Chavda, Jacqueline Findlay, Gisele Peirano, Katie Hopkins, Johann D D Pitout, Robert A Bonomo, Neil Woodford, Frank R DeLeo, Barry N Kreiswirth

Research output: Contribution to journalArticle (Academic Journal)peer-review

25 Citations (Scopus)

Abstract

We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.

Original languageEnglish
Pages (from-to)4196-9
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume58
Issue number7
DOIs
Publication statusPublished - Jul 2014

Keywords

  • Anti-Bacterial Agents
  • Bacterial Capsules
  • Bacterial Proteins
  • Carbapenems
  • DNA, Bacterial
  • Drug Resistance, Bacterial
  • Humans
  • Klebsiella pneumoniae
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Molecular Typing
  • Multiplex Polymerase Chain Reaction
  • beta-Lactamases
  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, U.S. Gov't, Non-P.H.S.

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