Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

Liang Chen; Kalyan D Chavda; Jacqueline Findlay; Gisele Peirano; Katie Hopkins; Johann D D Pitout; Robert A Bonomo; Neil Woodford; Frank R DeLeo; Barry N Kreiswirth

doi:10.1128/AAC.02673-14

Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

Liang Chen
, Kalyan D Chavda
, Jacqueline Findlay
, Gisele Peirano
, Katie Hopkins
, Johann D D Pitout
, Robert A Bonomo
, Neil Woodford
, Frank R DeLeo
, Barry N Kreiswirth

School of Cellular and Molecular Medicine

Research output: Contribution to journal › Article (Academic Journal) › peer-review

27 Citations (Scopus)

Abstract

We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.

Original language	English
Pages (from-to)	4196-9
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	58
Issue number	7
DOIs	https://doi.org/10.1128/AAC.02673-14
Publication status	Published - Jul 2014

Keywords

Anti-Bacterial Agents
Bacterial Capsules
Bacterial Proteins
Carbapenems
DNA, Bacterial
Drug Resistance, Bacterial
Humans
Klebsiella pneumoniae
Microbial Sensitivity Tests
Molecular Sequence Data
Molecular Typing
Multiplex Polymerase Chain Reaction
beta-Lactamases
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, U.S. Gov't, Non-P.H.S.

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Chen, L., Chavda, K. D., Findlay, J., Peirano, G., Hopkins, K., Pitout, J. D. D., Bonomo, R. A., Woodford, N., DeLeo, F. R., & Kreiswirth, B. N. (2014). Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains. Antimicrobial Agents and Chemotherapy, 58(7), 4196-9. https://doi.org/10.1128/AAC.02673-14

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title = "Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains",

abstract = "We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100\%) and specificity (100\%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.",

keywords = "Anti-Bacterial Agents, Bacterial Capsules, Bacterial Proteins, Carbapenems, DNA, Bacterial, Drug Resistance, Bacterial, Humans, Klebsiella pneumoniae, Microbial Sensitivity Tests, Molecular Sequence Data, Molecular Typing, Multiplex Polymerase Chain Reaction, beta-Lactamases, Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, Research Support, U.S. Gov't, Non-P.H.S.",

author = "Liang Chen and Chavda, \{Kalyan D\} and Jacqueline Findlay and Gisele Peirano and Katie Hopkins and Pitout, \{Johann D D\} and Bonomo, \{Robert A\} and Neil Woodford and DeLeo, \{Frank R\} and Kreiswirth, \{Barry N\}",

year = "2014",

month = jul,

doi = "10.1128/AAC.02673-14",

language = "English",

volume = "58",

pages = "4196--9",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

AU - Chen, Liang

AU - Chavda, Kalyan D

AU - Findlay, Jacqueline

AU - Peirano, Gisele

AU - Hopkins, Katie

AU - Pitout, Johann D D

AU - Bonomo, Robert A

AU - Woodford, Neil

AU - DeLeo, Frank R

AU - Kreiswirth, Barry N

PY - 2014/7

Y1 - 2014/7

N2 - We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.

AB - We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.

KW - Anti-Bacterial Agents

KW - Bacterial Capsules

KW - Bacterial Proteins

KW - Carbapenems

KW - DNA, Bacterial

KW - Drug Resistance, Bacterial

KW - Humans

KW - Klebsiella pneumoniae

KW - Microbial Sensitivity Tests

KW - Molecular Sequence Data

KW - Molecular Typing

KW - Multiplex Polymerase Chain Reaction

KW - beta-Lactamases

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, N.I.H., Intramural

KW - Research Support, U.S. Gov't, Non-P.H.S.

U2 - 10.1128/AAC.02673-14

DO - 10.1128/AAC.02673-14

M3 - Article (Academic Journal)

C2 - 24733470

SN - 0066-4804

VL - 58

SP - 4196

EP - 4199

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 7

ER -

Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

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Keywords

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