Murine studies and expressional analyses of human cardiac pericytes reveal novel trajectories of SARS-CoV-2 Spike protein-induced microvascular damage

Elisa Avolio; Prashant  Srivastava; Ji Jiahui; Michele Carrabba; Christopher Tze Wei Tsang; Yue Gu; Anita C Thomas; Kapil Gupta; Imre Berger; Costanza Emanueli; Paolo R Madeddu

doi:10.1038/s41392-023-01489-2

Murine studies and expressional analyses of human cardiac pericytes reveal novel trajectories of SARS-CoV-2 Spike protein-induced microvascular damage

Elisa Avolio, Prashant Srivastava, Ji Jiahui, Michele Carrabba, Christopher Tze Wei Tsang, Yue Gu, Anita C Thomas, Kapil Gupta, Imre Berger, Costanza Emanueli, Paolo R Madeddu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

2 Citations (Scopus)

38 Downloads (Pure)

Original language	English
Article number	232
Journal	Signal Transduction and Targeted Therapy
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41392-023-01489-2
Publication status	Published - 2 Jun 2023

Bibliographical note

Funding Information:
The authors wish to acknowledge the members of the University of Bristol COVID-19 Emergency Research Group (UNCOVER) for their scientific support. Drawings were generated with BioRender.com.

Funding Information:
This work was supported by the British Heart Foundation (BHF) project grant “Targeting the SARS-CoV-2 S-protein binding to the ACE2 receptor to preserve human cardiac pericytes function in COVID-19” (PG/20/10285) (to P.M. and E.A.); European Commission H2020 CORDIS project COVIRNA (project/id/101016072) (to C.E. and P.K.S.) and BHF Chair award (CH/15/1/31199) (to C.E). In addition, it was supported by a grant from the National Institute for Health Research (NIHR) Biomedical Research Centre at University Hospitals Bristol NHS Foundation Trust and the University of Bristol. E.A. is a postdoctoral researcher supported by the Heart Research UK translational project grant “Targeting pericytes for halting pulmonary hypertension in infants with congenital heart disease” (RG2697/21/23) (to P.M. and E.A.). I.B. is an investigator of the Wellcome Trust (106115/Z/14/Z).

Research Groups and Themes

Bristol Heart Institute

Keywords

Cardiac pericyte
COVID-19
Spike protein
microangiopathy
Transcriptomics

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2 Citations
1 Conference Contribution (Conference Proceeding)
1 Article (Academic Journal)
1 Special issue (Academic Journal)
1 Review article (Academic Journal)
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- 1 Other contribution
- 1 Discussion paper

The SARS-CoV-2 Spike protein: more than an entry key to heart damage
Avolio, E. & Madeddu, P. R., 2 Jun 2023, Nature Health Community blog: Springer Nature.
Research output: Working paper › Discussion paper

Open Access
The SARS-CoV-2 Spike protein modifies the transcriptional profile of human cardiac pericytes
Avolio, E., Madeddu, P. R., Srivastava, P. & Emanueli, C., 9 May 2023
Research output: Other contribution
Intravascular administration of soluble SARS-CoV-2 Spike protein triggers non-infective heart and lung inflammation and vascular pericytes rarefaction in healthy mice
Avolio, E., Carrabba, M., Gu, Y., Tsang, C. T. W., Amulic, B., Savitri, E. R., Still, K., Maggiani, F., Thomas, A. C., Groves, S. J., Gupta, K., Berger, I., Kafienah, W., Caputo, M. & Madeddu, P. R., 27 Jun 2022, (Accepted/In press) European Heart Journal Supplements.
Research output: Chapter in Book/Report/Conference proceeding › Conference Contribution (Conference Proceeding)

Cite this

Avolio, E., Srivastava, P., Jiahui, J., Carrabba, M., Tsang, C. T. W., Gu, Y., Thomas, A. C., Gupta, K., Berger, I., Emanueli, C., & Madeddu, P. R. (2023). Murine studies and expressional analyses of human cardiac pericytes reveal novel trajectories of SARS-CoV-2 Spike protein-induced microvascular damage. Signal Transduction and Targeted Therapy, 8(1), Article 232. https://doi.org/10.1038/s41392-023-01489-2

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keywords = "Cardiac pericyte, COVID-19, Spike protein, microangiopathy, Transcriptomics",

author = "Elisa Avolio and Prashant Srivastava and Ji Jiahui and Michele Carrabba and Tsang, {Christopher Tze Wei} and Yue Gu and Thomas, {Anita C} and Kapil Gupta and Imre Berger and Costanza Emanueli and Madeddu, {Paolo R}",

note = "Funding Information: The authors wish to acknowledge the members of the University of Bristol COVID-19 Emergency Research Group (UNCOVER) for their scientific support. Drawings were generated with BioRender.com. Funding Information: This work was supported by the British Heart Foundation (BHF) project grant “Targeting the SARS-CoV-2 S-protein binding to the ACE2 receptor to preserve human cardiac pericytes function in COVID-19” (PG/20/10285) (to P.M. and E.A.); European Commission H2020 CORDIS project COVIRNA (project/id/101016072) (to C.E. and P.K.S.) and BHF Chair award (CH/15/1/31199) (to C.E). In addition, it was supported by a grant from the National Institute for Health Research (NIHR) Biomedical Research Centre at University Hospitals Bristol NHS Foundation Trust and the University of Bristol. E.A. is a postdoctoral researcher supported by the Heart Research UK translational project grant “Targeting pericytes for halting pulmonary hypertension in infants with congenital heart disease” (RG2697/21/23) (to P.M. and E.A.). I.B. is an investigator of the Wellcome Trust (106115/Z/14/Z). ",

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Avolio, E, Srivastava, P, Jiahui, J, Carrabba, M, Tsang, CTW, Gu, Y, Thomas, AC, Gupta, K, Berger, I, Emanueli, C & Madeddu, PR 2023, 'Murine studies and expressional analyses of human cardiac pericytes reveal novel trajectories of SARS-CoV-2 Spike protein-induced microvascular damage', Signal Transduction and Targeted Therapy, vol. 8, no. 1, 232. https://doi.org/10.1038/s41392-023-01489-2

Murine studies and expressional analyses of human cardiac pericytes reveal novel trajectories of SARS-CoV-2 Spike protein-induced microvascular damage. / Avolio, Elisa; Srivastava, Prashant ; Jiahui, Ji et al.
In: Signal Transduction and Targeted Therapy, Vol. 8, No. 1, 232, 02.06.2023.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

T1 - Murine studies and expressional analyses of human cardiac pericytes reveal novel trajectories of SARS-CoV-2 Spike protein-induced microvascular damage

AU - Avolio, Elisa

AU - Srivastava, Prashant

AU - Jiahui, Ji

AU - Carrabba, Michele

AU - Tsang, Christopher Tze Wei

AU - Gu, Yue

AU - Thomas, Anita C

AU - Gupta, Kapil

AU - Berger, Imre

AU - Emanueli, Costanza

AU - Madeddu, Paolo R

N1 - Funding Information: The authors wish to acknowledge the members of the University of Bristol COVID-19 Emergency Research Group (UNCOVER) for their scientific support. Drawings were generated with BioRender.com. Funding Information: This work was supported by the British Heart Foundation (BHF) project grant “Targeting the SARS-CoV-2 S-protein binding to the ACE2 receptor to preserve human cardiac pericytes function in COVID-19” (PG/20/10285) (to P.M. and E.A.); European Commission H2020 CORDIS project COVIRNA (project/id/101016072) (to C.E. and P.K.S.) and BHF Chair award (CH/15/1/31199) (to C.E). In addition, it was supported by a grant from the National Institute for Health Research (NIHR) Biomedical Research Centre at University Hospitals Bristol NHS Foundation Trust and the University of Bristol. E.A. is a postdoctoral researcher supported by the Heart Research UK translational project grant “Targeting pericytes for halting pulmonary hypertension in infants with congenital heart disease” (RG2697/21/23) (to P.M. and E.A.). I.B. is an investigator of the Wellcome Trust (106115/Z/14/Z).

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KW - Cardiac pericyte

KW - COVID-19

KW - Spike protein

KW - microangiopathy

KW - Transcriptomics

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ER -

Murine studies and expressional analyses of human cardiac pericytes reveal novel trajectories of SARS-CoV-2 Spike protein-induced microvascular damage

Bibliographical note

Research Groups and Themes

Keywords

Access to Document

Handle.net

Research output

The SARS-CoV-2 Spike protein: more than an entry key to heart damage

The SARS-CoV-2 Spike protein modifies the transcriptional profile of human cardiac pericytes

Intravascular administration of soluble SARS-CoV-2 Spike protein triggers non-infective heart and lung inflammation and vascular pericytes rarefaction in healthy mice

Cite this