Neisseria meningitidis Opc invasin binds to the cytoskeletal protein alpha-actinin

Claudia Sa E. Cunha, Natalie J. Griffiths, Isabel Murillo Cabeza, Mumtaz Virji*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

23 Citations (Scopus)

Abstract

Neisseria meningitidis Opc protein is an effective invasin for human endothelial cells. We have investigated novel human endothelial receptors targeted by Opc and observed that Opc-expressing bacteria interacted with a 100 kDa protein in whole-cell lysates of human endothelial and epithelial cells. The identity of the protein was established as alpha-actinin by mass spectrometry. Opc expression was essential for the recognition of alpha-actinin whether provided in a purified form or in cell extracts. The interaction of the two proteins did not involve intermediate molecules. As there was no demonstrable expression of alpha-actinin on the surfaces of any of the eight cell lines studied, the likelihood of the interactions after meningococcal internalization was examined. Confocal imaging demonstrated considerable colocalization of N. meningitidis with alpha-actinin especially after a prolonged period of internalization. This may imply that bacteria and alpha-actinin initially occur in separate compartments and co-compartmentalization occurs progressively over the 8 h infection period used. In conclusion, these studies have identified a novel and an intracellular target for the N. meningitidis Opc invasin. Since alpha-actinin is a modulator of a variety of signalling pathways and of cytoskeletal functions, its targeting by Opc may enable bacteria to survive/translocate across endothelial barriers.

Translated title of the contributionNeisseria meningitidis Opc invasin binds to the cytoskeletal protein alpha-actinin
Original languageEnglish
Pages (from-to)389 - 405
Number of pages17
JournalCellular Microbiology
Volume11
Issue number3
DOIs
Publication statusPublished - Mar 2009

Bibliographical note

Publisher: Wiley-Blackwell Publishing Inc

Keywords

  • MICROVASCULAR ENDOTHELIAL-CELLS
  • OUTER-MEMBRANE PROTEIN
  • EPITHELIAL-CELLS
  • PROTEOGLYCAN RECEPTORS
  • CRITICAL DETERMINANTS
  • PATHOGENIC NEISSERIA
  • IGA1 PROTEASE
  • IN-VITRO
  • GONORRHOEAE
  • EXPRESSION

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