Neuronal overexpression of Alzheimer's disease and down's syndrome associated DYRK1A/minibrain gene alters motor decline, neurodegeneration and synaptic plasticity in Drosophila

Simon A. Lowe, Maria M. Usowicz*, James J.L. Hodge

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

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Abstract

Down syndrome (DS) is characterised by abnormal cognitive and motor development, and later in life by progressive Alzheimer's disease (AD)-like dementia, neuropathology, declining motor function and shorter life expectancy. It is caused by trisomy of chromosome 21 (Hsa21), but how individual Hsa21 genes contribute to various aspects of the disorder is incompletely understood. Previous work has demonstrated a role for triplication of the Hsa21 gene DYRK1A in cognitive and motor deficits, as well as in altered neurogenesis and neurofibrillary degeneration in the DS brain, but its contribution to other DS phenotypes is unclear. Here we demonstrate that overexpression of minibrain (mnb), the Drosophila ortholog of DYRK1A, in the Drosophila nervous system accelerated age-dependent decline in motor performance and shortened lifespan. Overexpression of mnb in the eye was neurotoxic and overexpression in ellipsoid body neurons in the brain caused age-dependent neurodegeneration. At the larval neuromuscular junction, an established model for mammalian central glutamatergic synapses, neuronal mnb overexpression enhanced spontaneous vesicular transmitter release. It also slowed recovery from short-term depression of evoked transmitter release induced by high-frequency nerve stimulation and increased the number of boutons in one of the two glutamatergic motor neurons innervating the muscle. These results provide further insight into the roles of DYRK1A triplication in abnormal aging and synaptic dysfunction in DS.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalNeurobiology of Disease
Volume125
Early online date28 Jan 2019
DOIs
Publication statusPublished - May 2019

Keywords

  • Alzheimer's disease
  • Down's syndrome
  • Drosophila
  • DYRK1A/minibrain
  • Motor decline
  • Neurodegeneration
  • Neuromuscular junction
  • Short-term synaptic depression
  • Spontaneous vesicular transmitter release
  • Synaptic transmission

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